IL-13R alpha 2 Recombinant Rabbit Monoclonal Antibody [PSH03-85]

☑ Relative expression (RE)

Western blot analysis of IL-13R alpha 2 on different lysates with Rabbit anti-IL-13R alpha 2 antibody (HA722051) at 1/2,000 dilution.

Lane 1: A375 cell lysate (no heat)
Lane 2: U-937 cell lysate (no heat) (negative)
Lane 3: Daudi cell lysate (negative)

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 44 kDa
Observed band size: 50-80 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722051) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of IL-13R alpha 2 on U-87 MG cell lysates with Rabbit anti-IL-13R alpha 2 antibody (HA722051) at 1/2,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 44 kDa
Observed band size: 50-70 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722051) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-IL-13R alpha 2 antibody (HA722051) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722051) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Relative expression (RE)

Flow cytometric analysis of A375 (positive) and U-937 (negative) cells labeling IL-13R alpha 2.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722051, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).