IRE1 Mouse Monoclonal Antibody [C9-F2]
Catalog# EM1708-88
IRE1 Mouse Monoclonal Antibody [C9-F2]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
IRE1 Mouse Monoclonal Antibody [C9-F2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
110 kDa
Positive Control
Raji, A431, Jurkat, Hela, HEK293 cell lysate, human placenta tissue, human brain tissue, human stomach tissue.
Conjugation
unconjugated
Clone Number
C9-F2
Product Features
Form
Liquid
Concentration
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
Target
Function
The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1α and IRE1β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes. In response to stress, sensors for the ER mammalian cells activate IRE1α and IRE1β, which then results in the phosphorylation of JNK (Jun N-Terminal Kinase) and the activation of the cellular MAP kinase pathway.
Background References
暂无
Subcellular Location
Endoplasmic reticulum membrane.
UNIPROT
Synonyms
Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
Endoplasmic reticulum to nucleus signaling 1 antibody
Endoplasmic reticulum-to-nucleus signaling 1 antibody
Endoribonuclease antibody
ER to nucleus signaling 1 antibody
ERN 1 antibody
Ern1 antibody
ERN1_HUMAN antibody
hIRE 1p antibody
hIRE1p antibody
ExpandImages
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Western blot analysis of ERN1 on different cell lysate using anti-ERN1 antibody at 1/1,000 dilution.
Positive control:
Line1: Raji
Line2: A431
Line3: Jurkat
Line4: Hela
Line5: HEK293 -
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERN1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ERN1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-ERN1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60]
Application: WB
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
Products with the same target and pathway
IRE1 Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60]
Application: WB
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
