FOXP3 Recombinant Rabbit Monoclonal Antibody
Catalog# IRS053
FOXP3 Recombinant Rabbit Monoclonal Antibody
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mIHC
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Mouse
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unconjugated
Overview
Product Name
FOXP3 Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human FOXP3 aa 101-370.
Species Reactivity
Mouse
Validated Applications
mIHC
Molecular Weight
Predicted band size: 47 kDa
Positive Control
Mouse spleen tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
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1:100
Target
Function
FOXP3 (forkhead box P3), also known as scurfin, is a protein involved in immune system responses. A member of the FOX protein family, FOXP3 appears to function as a master regulator of the regulatory pathway in the development and function of regulatory T cells. Regulatory T cells generally turn the immune response down. In cancer, an excess of regulatory T cell activity can prevent the immune system from destroying cancer cells. In autoimmune disease, a deficiency of regulatory T cell activity can allow other autoimmune cells to attack the body's own tissues.
Background References
1. Wang J et al. Human FOXP3 and tumour microenvironment. Immunology. 2023 Feb
2. Ziółkowska-Suchanek I et al. FOXP3: A Player of Immunogenetic Architecture in Lung Cancer. Genes (Basel). 2024 Apr
Subcellular Location
Nucleus, Cytoplasm.
UNIPROT
Synonyms
AIID antibody
DIETER antibody
Forkhead box P3 antibody
Forkhead box protein P3 antibody
FOXP3 antibody
FOXP3_HUMAN antibody
FOXP3delta7 antibody
Immune dysregulation polyendocrinopathy enteropathy X linked antibody
Immunodeficiency polyendocrinopathy enteropathy X linked antibody
IPEX antibody
ExpandImages
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mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FOXP3 antibody (IRS053) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1 (IRS059), F4/80 (IRS052), FOXP3 (IRS053), CD11c and CD4 (IRS051) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"