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HNF-4-alpha Recombinant Rabbit Monoclonal Antibody

Usd: 209

Specification

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Catalog# IRS055

HNF-4-alpha Recombinant Rabbit Monoclonal Antibody

Application

  • mIHC

Reactivity

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

HNF-4-alpha Recombinant Rabbit Monoclonal Antibody

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse HNF-4-alpha aa 1-250/474.

Species Reactivity

Mouse

Validated Applications

mIHC

Molecular Weight

Predicted band size: 53 kDa

Positive Control

Mouse liver tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • mIHC

  • 1:100

Target

Function

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms.

Background References

1. Song H. et. al. HNF4A-AS1/hnRNPU/CTCF axis as a therapeutic target for aerobic glycolysis and neuroblastoma progression. J Hematol Oncol. 2020 Mar

2. Zhang X. et. al. circRNA_104075 stimulates YAP-dependent tumorigenesis through the regulation of HNF4a and may serve as a diagnostic marker in hepatocellular carcinoma. Cell Death Dis. 2018 Oct

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P49698 Mouse

Synonyms

FLJ39654 antibody

FRTS4 antibody

Hepatic nuclear factor 4 alpha antibody

Hepatocyte nuclear factor 4 alpha antibody

Hepatocyte nuclear factor 4 antibody

Hepatocyte nuclear factor 4-alpha antibody

HNF 4 alpha antibody

HNF 4 antibody

HNF-4-alpha antibody

HNF4 antibody

Expand

Images

  • mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with HNF-4-α (<a href="/products/IRS055" style="font-weight: bold;text-decoration: underline;">IRS055</a>), GS and β Catenin (<a href="/products/IRS061" style="font-weight: bold;text-decoration: underline;">IRS061</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with HNF-4-α (IRS055), GS and β Catenin (IRS061) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-HNF-4-alpha antibody (<a href="/products/IRS055" style="font-weight: bold;text-decoration: underline;">IRS055</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-HNF-4-alpha antibody (IRS055) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"