Olig2 Recombinant Rabbit Monoclonal Antibody
Catalog# IRS067
Olig2 Recombinant Rabbit Monoclonal Antibody
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mIHC
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Mouse
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unconjugated
Overview
Product Name
Olig2 Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Olig2 aa 238-287 / 323.
Species Reactivity
Mouse
Validated Applications
mIHC
Molecular Weight
Predicted band size: 32 kDa
Positive Control
Mouse brain tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
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1:100
Target
Function
The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors include OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG2 is more widely expressed in embryonic brain than OLIG1, while OLIG3 is primarily expressed in non-neural tissues. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.
Background References
1. Kiely AP et al. a-Synucleinopathy associated with G51D SNCA mutation: a link between Parkinson\'s disease and multiple system atrophy Acta Neuropathol 125:753-69 (2013).
2. Wang K et al. Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells. Cloning Stem Cells 11:483-96 (2009).
Tissue Specificity
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
Subcellular Location
Nucleus, Cytoplasm.
UNIPROT
Synonyms
Basic domain helix loop helix protein class B 1 antibody
Basic helix loop helix protein class B 1 antibody
BHLHB antibody
bHLHB1 antibody
bHLHe19 antibody
Class B basic helix loop helix protein 1 antibody
Class B basic helix-loop-helix protein 1 antibody
class E basic helix loop helix protein 19 antibody
Class E basic helix-loop-helix protein 19 antibody
Human protein kinase C binding protein RACK17 antibody
ExpandImages
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mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with SOX2, TBR1 (IRS070), Olig2 (IRS067), CD34 (IRS050) and GFAP (IRS069) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Olig2 antibody (IRS067) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"