Aquaporin 2 Recombinant Rabbit Monoclonal Antibody
Catalog# IRS089RB
Aquaporin 2 Recombinant Rabbit Monoclonal Antibody
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mIHC
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Mouse
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unconjugated
Overview
Product Name
Aquaporin 2 Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Aquaporin 2 aa 215-263/271
Species Reactivity
Mouse
Validated Applications
mIHC
Target Molecular Weight
Predicted band size: 29 kDa
Positive Control
Mouse kidney tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
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1:100
Target
Function
Aquaporin-2 (AQP-2) is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. It is encoded by the AQP2 gene.It is the only aquaporin regulated by vasopressin.The basic job of aquaporin 2 is to reabsorb water from the urine while its being removed from the blood by the kidney. Aquaporin 2 is in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed, vasopressin binds to the cell surface vasopressin receptor thereby activating a signaling pathway that causes the aquaporin 2 containing vesicles to fuse with the plasma membrane, so the aquaporin 2 can be used by the cell.
Background References
1. Valenti G, Tamma G. The vasopressin-aquaporin-2 pathway syndromes. Handb Clin Neurol. 2021;181:249-259. doi: 10.1016/B978-0-12-820683-6.00018-X. PMID: 34238461.
2. Olesen ET, Fenton RA. Aquaporin-2 membrane targeting: still a conundrum. Am J Physiol Renal Physiol. 2017 Apr 1;312(4):F744-F747. doi: 10.1152/ajprenal.00010.2017. Epub 2017 Feb 8. PMID: 28179252.
Subcellular Location
Apical cell membrane, Basolateral cell membrane, Cell membrane, Cytoplasmic vesicle membrane, Golgi apparatus, trans-Golgi network membrane
UNIPROT
Synonyms
ADH water channel antibody
AQP 2 antibody
AQP CD antibody
AQP-2 antibody
AQP-CD antibody
AQP2 antibody
AQP2_HUMAN antibody
AQPCD antibody
Aquaporin 2 collecting duct antibody
Aquaporin CD antibody
ExpandImages
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mIHC analysis of mouse kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Aquaporin 2 antibody (IRS089RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"