GFP Recombinant Rabbit Monoclonal Antibody
Catalog# IRS091
GFP Recombinant Rabbit Monoclonal Antibody
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mIHC
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Species independent
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unconjugated
Overview
Product Name
GFP Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Aequorea victoria GFP aa 1-50 / 238.
Species Reactivity
Species independent
Validated Applications
mIHC
Molecular Weight
Predicted band size: 27 kDa
Positive Control
GFP-FLK transgenic zebrafish brain blood vessels.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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mIHC
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1:100
Target
Function
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
Background References
1. Yu, H. et al. 2016. AAV-Mediated Gene Transfer to Dorsal Root Ganglion. Methods in molecular biology (Clifton, N.J.). 1382: 251-61.
2. Yamaoka, M. et al. 2016. PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a. J. Cell. Sci. 129: 637-49.
Sequence Similarity
Belongs to the GFP family.
Tissue Specificity
Photocytes.
Post-translational Modification
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Synonyms
GFP antibody
Green fluorescent protein antibody
yfp antibody
Images
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mIHC analysis of GFP-FLK transgenic zebrafish brain blood vessels (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-GFP antibody (IRS091) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"