Cytokeratin 19 Recombinant Antibody - Rat IgG1 (Chimeric)
Catalog# IRS134RT
Cytokeratin 19 Recombinant Antibody - Rat IgG1 (Chimeric)
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mIHC
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Human
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unconjugated
Overview
Product Name
Cytokeratin 19 Recombinant Antibody - Rat IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human Cytokeratin 19 aa 348-400 / 400.
Species Reactivity
Human
Validated Applications
mIHC
Molecular Weight
Predicted band size: 44 kDa
Positive Control
Human tonsil tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
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1:100
Target
Function
Keratin, type I cytoskeletal 19 also known as cytokeratin-19 (CK-19) or keratin-19 (K19) is a 40 kDa protein that in humans is encoded by the KRT19 gene. Keratin 19 is a type I keratin. Keratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically found in the periderm, the transiently superficial layer that envelops the developing epidermis.
Background References
1. Guye P et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016).
2. Cui M et al. PTEN is a potent suppressor of small cell lung cancer. Mol Cancer Res 12:654-9 (2014).
Subcellular Location
Cytoplasm
UNIPROT
Synonyms
40 kDa keratin intermediate filament antibody
CK 19 antibody
CK-19 antibody
CK19 antibody
Cytokeratin 19 antibody
Cytokeratin-19 antibody
K19 antibody
K1C19_HUMAN antibody
K1CS antibody
Keratin 19 antibody
ExpandImages
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mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rat anti-Cytokeratin 19 antibody (IRS134RT) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900810). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"