CYP2E1 Recombinant Rabbit Monoclonal Antibody
Catalog# IRS139RB
CYP2E1 Recombinant Rabbit Monoclonal Antibody
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mIHC
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Human
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unconjugated
Overview
Product Name
CYP2E1 Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CYP2E1 aa 71-114 / 439.
Species Reactivity
Human
Validated Applications
mIHC
Molecular Weight
Predicted band size: 57 kDa
Positive Control
Human liver tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
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1:100
Target
Function
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) .Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable).
Background References
1. Gi M et al. Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-Dichloropropane in Male Syrian Hamsters and B6C3F1 Mice. Toxicol Sci 145:196-208 (2015).
2. Boussadia B et al. Effect of status epilepticus and antiepileptic drugs on CYP2E1 brain expression. Neuroscience 281C:124-134 (2014).
Subcellular Location
Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane.
UNIPROT
Synonyms
4-nitrophenol 2-hydroxylase antibody
CP2E1_HUMAN antibody
CPE1 antibody
CYP2E antibody
Cyp2e1 antibody
CYPIIE1 antibody
Cytochrome P450 2E1 antibody
Cytochrome P450 isozyme 3A antibody
Cytochrome P450 LM3A antibody
Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody
ExpandImages
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mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CYP2E1 antibody (IRS139RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"