Hormone sensitive lipase / HSL Recombinant Rabbit Monoclonal Antibody
Catalog# IRS157RB
Hormone sensitive lipase / HSL Recombinant Rabbit Monoclonal Antibody
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mIHC
-
Human
-
unconjugated
Overview
Product Name
Hormone sensitive lipase / HSL Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human HSL aa 931-980.
Species Reactivity
Human
Validated Applications
mIHC
Molecular Weight
Predicted band size: 117 kDa
Positive Control
Human breast tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
-
1:100
Target
Function
The main function of hormone-sensitive lipase is to mobilize stored fats. HSL functions to hydrolyze either a fatty acid from a triacylglycerol molecule, freeing a fatty acid and diglyceride, or a fatty acid from a diacylglycerol molecule, freeing a fatty acid and monoglyceride. This process allows energy metabolism in mammals. Although HSL is able to catalyze hydrolysis of triglycerides and diglycerides, another enzyme found in adipose tissue, adipose triglyceride lipase (ATGL), has a higher affinity for triglycerides than HSL, and ATGL predominantly acts as the enzyme for triglyceride-specific hydrolysis in the adipocyte. Hormone-sensitive lipase, which has 11-fold greater affinity for diglycerides than triglycerides, predominantly cleaves these diglycerides, forming 2-monoglyceride and a free fatty acid.
Background References
1. Althaher AR. An Overview of Hormone-Sensitive Lipase (HSL). ScientificWorldJournal. 2022 Dec
2. Shen WJ et al. Hormone sensitive lipase ablation promotes bone regeneration. Biochim Biophys Acta Mol Basis Dis. 2022 Sep
Subcellular Location
Cell membrane, Membrane, caveola, Cytoplasm, cytosol, Lipid droplet.
UNIPROT
Synonyms
Hormone sensitive lipase antibody
Hormone sensitive lipase testicular isoform antibody
Hormone-sensitive lipase antibody
HSL antibody
LHS antibody
Lipase hormone sensitive antibody
LIPE antibody
LIPS_HUMAN antibody
Images
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mIHC analysis of human breast tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Hormone sensitive lipase / HSL antibody (IRS157RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Hormone sensitive lipase / HSL Recombinant Rabbit Monoclonal Antibody [PSH07-22]
Application: WB,IHC-P,IP,mIHC,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
