Occludin Recombinant Rabbit Monoclonal Antibody
Catalog# IRS183RB
Occludin Recombinant Rabbit Monoclonal Antibody
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mIHC
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Mouse
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unconjugated
Overview
Product Name
Occludin Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within N-terminal human Occludin.
Species Reactivity
Mouse
Validated Applications
mIHC
Molecular Weight
Predicted band size: 59 kDa
Positive Control
Mouse small intestine tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
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1:100
Target
Function
Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by poly-unsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.
Background References
1. Cao S et al. Hydrogen sulfide attenuates brain edema in early brain injury after subarachnoid hemorrhage in rats: Possible involvement of MMP-9 induced blood-brain barrier disruption and AQP4 expression. Neurosci Lett 621:88-97 (2016).
2. Zhang C et al. Catalpol downregulates vascular endothelial-cadherin expression and induces vascular hyperpermeability. Mol Med Rep 13:373-8 (2016).
Subcellular Location
Cell membrane, tight junction.
UNIPROT
Synonyms
BLCPMG antibody
FLJ08163 antibody
FLJ18079 antibody
FLJ77961 antibody
FLJ94056 antibody
MGC34277 antibody
Occludin antibody
Ocln antibody
OCLN_HUMAN antibody
Tight junction protein occludin antibody
Images
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mIHC analysis of mouse small intestine tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Occludin antibody (IRS183RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"