pan Cytokeratin Recombinant Antibody - Rabbit IgG (Chimeric)
Catalog# IRS186RB
pan Cytokeratin Recombinant Antibody - Rabbit IgG (Chimeric)
-
mIHC
-
Human
-
unconjugated
Overview
Product Name
pan Cytokeratin Recombinant Antibody - Rabbit IgG (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Full length native protein corresponding to Human pan Cytokeratin.
Species Reactivity
Human
Validated Applications
mIHC
Positive Control
Human tonsil tissue, human lung squamous cell carcinoma tissue, human liver tissue, human intrahepatic bile duct carcinoma tissue, human gastric cancer tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
-
1:100
Target
Function
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Since each epithelium contains at least one acidic and one basic keratin, this antibody is used to observe the distribution of keratin-containing cells in normal epithelia and to identify neoplasms derived from such epithelium. Recognizes humancytokeratins 10, 14, 15, 16 and 19, and cytokeratins 1, 2, 3, 4, 5, 6, 7 and 8.
Background References
暂无
Subcellular Location
Cytoplasmic
UNIPROT
Synonyms
pan Cytokeratin antibody
pan-Cytokeratin antibody
pan ck antibody
pan-ck antibody
panck antibody
pan Keratin antibody
Images
-
mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-pan Cytokeratin antibody (IRS186RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"