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PD1 Mouse Monoclonal Antibody

Usd: 278

Specification

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Catalog# IRS190MS

PD1 Mouse Monoclonal Antibody

Application

  • mIHC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PD1 Mouse Monoclonal Antibody

Antibody Type

Mouse Monoclonal Antibody

Species Reactivity

Human

Validated Applications

mIHC

Target Molecular Weight

Predicted band size: 32 kDa

Positive Control

Human tonsil tissue, human spleen tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Application Dilution

  • mIHC

  • 1:100

Target

Function

Programmed cell death protein 1 (PD-1), (CD279 cluster of differentiation 279). PD-1 is a protein encoded in humans by the PDCD1 gene. PD-1 is a cell surface receptor on T cells and B cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells. PD-1 is an immune checkpoint and guards against autoimmunity through two mechanisms. First, it promotes apoptosis (programmed cell death) of antigen-specific T-cells in lymph nodes. Second, it reduces apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells). PD-1 inhibitors, a new class of drugs that block PD-1, activate the immune system to attack tumors and are used to treat certain types of cancer. PD-1 is a cell surface receptor that belongs to the immunoglobulin superfamily and is expressed on T cells and pro-B cells. PD-1 binds two ligands, PD-L1 and PD-L2.

Background References

1. Lei Q et al. Resistance Mechanisms of Anti-PD1/PDL1 Therapy in Solid Tumors. Front Cell Dev Biol. 2020 Jul

2. Chamoto K et al. Insights from a 30-year journey: function, regulation and therapeutic modulation of PD1. Nat Rev Immunol. 2023 Oct

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q15116 Human

Synonyms

CD279 antibody

CD279 antigen antibody

hPD 1 antibody

hPD l antibody

hPD-1 antibody

hSLE1 antibody

PD 1 antibody

PD-1 antibody

PD1 antibody

PDCD 1 antibody

Expand

Images

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (<a href="/products/IRS190MS" style="font-weight: bold;text-decoration: underline;">IRS190MS</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900809" style="font-weight: bold;text-decoration: underline;">900809</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (IRS190MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (<a href="/products/IRS190MS" style="font-weight: bold;text-decoration: underline;">IRS190MS</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900809" style="font-weight: bold;text-decoration: underline;">900809</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (IRS190MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (<a href="/products/IRS190MS" style="font-weight: bold;text-decoration: underline;">IRS190MS</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900809" style="font-weight: bold;text-decoration: underline;">900809</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (IRS190MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (<a href="/products/IRS190MS" style="font-weight: bold;text-decoration: underline;">IRS190MS</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900809" style="font-weight: bold;text-decoration: underline;">900809</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-PD1 antibody (IRS190MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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