Podoplanin Recombinant Rabbit Monoclonal Antibody
Catalog# IRS194RB
Podoplanin Recombinant Rabbit Monoclonal Antibody
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mIHC
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Human
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unconjugated
Overview
Product Name
Podoplanin Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Podoplanin aa 35-75 / 162.
Species Reactivity
Human
Validated Applications
mIHC
Molecular Weight
Predicted band size: 17 kDa
Positive Control
Human breast tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
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1:100
Target
Function
Puromycin aminonucleoside nephrosis (PAN) is a rat model for human minimal change nephropathy. During PAN, severe proteinuria is induced that is paralleled by a reduced expression of a rat podocyte protein, named podoplanin. Podoplanin, also known as glycoprotein 38 (gp38) is a type I membrane protein. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes.
Background References
1. Xu Y et al. Polypeptide N-Acetylgalactosaminyltransferase 13 Contributes to Neurogenesis via Stabilizing the Mucin-type O-Glycoprotein Podoplanin. J Biol Chem 291:23477-23488 (2016).
2. Xia Y et al. Podoplanin associates with adverse postoperative prognosis of patients with clear cell renal cell carcinoma. Cancer Sci 107:1243-9 (2016).
Subcellular Location
Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane.
UNIPROT
Synonyms
Aggrus antibody
Glycoprotein 36 KD antibody
Glycoprotein 36 antibody
gp 36 antibody
GP 38 antibody
GP 40 antibody
gp36 antibody
GP38 antibody
GP40 antibody
HT1A 1 antibody
ExpandImages
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mIHC analysis of human breast tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Podoplanin antibody (IRS194RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"