Slow Skeletal Myosin Heavy chain Recombinant Rabbit Monoclonal Antibody
Catalog# IRS201RB
Slow Skeletal Myosin Heavy chain Recombinant Rabbit Monoclonal Antibody
-
mIHC
-
Mouse
-
unconjugated
Overview
Product Name
Slow Skeletal Myosin Heavy chain Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human MYH7 aa 1,250-1,300.
Species Reactivity
Mouse
Validated Applications
mIHC
Molecular Weight
Predicted band size: 223 kDa
Positive Control
Mouse muscle tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
-
1:100
Target
Function
MYH7 is a gene encoding a myosin heavy chain beta (MHC-β) isoform (slow twitch) expressed primarily in the heart, but also in skeletal muscles (type I fibers). This isoform is distinct from the fast isoform of cardiac myosin heavy chain, MYH6, referred to as MHC-α. MHC-β is the major protein comprising the thick filament that forms the sarcomeres in cardiac muscle and plays a major role in cardiac muscle contraction.
Background References
1. Gao Y et al. MYH7 in cardiomyopathy and skeletal muscle myopathy. Mol Cell Biochem. 2024 Feb
2. Park J et al. A genome-first approach to rare variants in hypertrophic cardiomyopathy genes MYBPC3 and MYH7 in a medical biobank. Hum Mol Genet. 2022 Mar
Subcellular Location
Cytoplasm, myofibril, sarcomere.
UNIPROT
Synonyms
Beta myosin heavy chain antibody
cardiac muscle beta isoform antibody
CMD1S antibody
CMH1 antibody
MPD1 antibody
MYH7 antibody
MYH7_HUMAN antibody
Myhc slow antibody
MyHC-beta antibody
MyHC-slow antibody
ExpandImages
-
mIHC analysis of mouse muscle tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (IRS201RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"