Glutamine Synthetase Recombinant Mouse Monoclonal Antibody
Catalog# IRS231MS
Glutamine Synthetase Recombinant Mouse Monoclonal Antibody
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mIHC
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Mouse
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unconjugated
Overview
Product Name
Glutamine Synthetase Recombinant Mouse Monoclonal Antibody
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human Glutamine synthetase aa 190-373.
Species Reactivity
Mouse
Validated Applications
mIHC
Molecular Weight
Predicted band size: 42 kDa
Positive Control
Mouse liver tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
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1:100
Target
Function
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.
Background References
1. Muthu M. et. al. GLUL Ablation Can Confer Drug Resistance to Cancer Cells via a Malate-Aspartate Shuttle-Mediated Mechanism. Cancers (Basel). 2019 Dec
2. Wang Y. et. al. GLUL Promotes Cell Proliferation in Breast Cancer. J Cell Biochem. 2017 Aug
Subcellular Location
Microsome, Cytosol, Mitochondrion, Cell membrane.
UNIPROT
Synonyms
cell proliferation-inducing protein 59 antibody
Cgl2214 antibody
GLNA antibody
GLNA_HUMAN antibody
GLNS antibody
GLUL antibody
Glutamate ammonia ligase antibody
Glutamate decarboxylase antibody
Glutamate--ammonia ligase antibody
glutamine synthase antibody
ExpandImages
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mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-Glutamine Synthetase antibody (IRS231MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"