CD21 Recombinant Antibody - Rat IgG1 (Chimeric)
Catalog# IRS265RT
CD21 Recombinant Antibody - Rat IgG1 (Chimeric)
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mIHC
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Human
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unconjugated
Overview
Product Name
CD21 Recombinant Antibody - Rat IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human CD21 aa 1000 to the C-terminus.
Species Reactivity
Human
Validated Applications
mIHC
Molecular Weight
Predicted band size: 113 kDa
Positive Control
Human tonsils tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
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1:100
Target
Function
CD21 is a type I integral membrane glycoprotein that serves as a receptor for the C3d complement fragment and for the Epstein-Barr virus. It plays a role in B cell activation and proliferation and undergoes phosphorylation after B cell activation with phorbol esters. CD21 is expressed on mature B cells, follicular dendritic cells, pharyngeal and cervical epithelial cells and a subset of thymocytes. The adaptive immune response is tightly regulated to limit responding cells in an antigen-specific manner. On B cells, co-receptors CD21/CD19 modulate the strength of B cell Ag receptor (BCR) signals, thereby influencing cell fate. Complement receptor (CR) type 2 (CR2/ CD21) is normally expressed during the immature and mature stages of B cell development. In association with CD19, CD21 plays an important role in enhancing mature B cell responses to foreign antigens.
Background References
1. Smith NA. et. al. CD21 (Complement Receptor 2) Is the Receptor for Epstein-Barr Virus Entry into T Cells. J Virol. 2020 May
2. Visentini M. et. al. CD21(low) B cells are predictive markers of new digital ulcers in systemic sclerosis. Clin Exp Immunol. 2021 Aug
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
C3DR antibody
CD 21 antibody
CD21 antibody
Complement C3d receptor 2 antibody
Complement C3d receptor antibody
Complement component (3d/Epstein Barr virus) receptor 2 antibody
Complement receptor type 2 antibody
CR antibody
Cr2 antibody
CR2_HUMAN antibody
ExpandImages
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mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rat anti-CD21 antibody (IRS265RT) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900810). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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