Skip to content

E-Cadherin Recombinant Antibody - Rat IgG1 (Chimeric)

Usd: 209

Specification

Bulk Order Quote | Customization Request

Catalog# IRS284RT

E-Cadherin Recombinant Antibody - Rat IgG1 (Chimeric)

Application

  • mIHC

Reactivity

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

E-Cadherin Recombinant Antibody - Rat IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within mouse E-Cadherin aa 350-550.

Species Reactivity

Mouse

Validated Applications

mIHC

Molecular Weight

Predicted band size: 98 kDa

Positive Control

Mouse pancreas tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • mIHC

  • 1:100

Target

Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Background References

1. Thomas E Meigs et al. Galpha12 and Galpha13 negatively regulate the adhesive functions of cadherin. J Biol Chem 277(27):24594-600 (2002)

2. Georgia Agiostratidou et al. The cytoplasmic sequence of E-cadherin promotes non-amyloidogenic degradation of A beta precursors. 96(4):1182-8 (2006)

Subcellular Location

Cell membrane, Endosome, Golgi apparatus.

UNIPROT

SwissProt: P09803 Mouse

Synonyms

Cadherin-1

CAM 120/80

Epithelial cadherin (E-cadherin)

Uvomorulin

Images

  • mIHC analysis of mouse pancreas tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rat anti-E-Cadherin antibody (<a href="/products/IRS284RT" style="font-weight: bold;text-decoration: underline;">IRS284RT</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900810" style="font-weight: bold;text-decoration: underline;">900810</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of mouse pancreas tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rat anti-E-Cadherin antibody (IRS284RT) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900810). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"