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iFluor™ 488 Conjugated Iba1 Recombinant Rabbit Monoclonal Antibody [JM36-62]

Usd: 429

Specification

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Catalog# HA720158F

iFluor™ 488 Conjugated Iba1 Recombinant Rabbit Monoclonal Antibody [JM36-62]

Application

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • iFluor™ 488

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

iFluor™ 488 Conjugated Iba1 Recombinant Rabbit Monoclonal Antibody [JM36-62]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within N-terminal human Iba1.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IF-Cell, FC

Molecular Weight

Predicted band size: 17 kDa

Positive Control

SH-SY5Y, RAW264.7, J774A.1.

Conjugation

iFluor™ 488

Clone Number

JM36-62

RRID

AB_3072096

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Cell

  • 1:100-1:250

  • FC

  • 1:500-1:1,000

Target

Function

Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).

Background References

1. Hennessy E et al. Systemic TNF-a produces acute cognitive dysfunction and exaggerated sickness behavior when superimposed upon progressive neurodegeneration. Brain Behav Immun 59:233-244 (2017).

2. Arentsen T et al. The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior. Mol Psychiatry 22:257-266 (2017).

Subcellular Location

Cytoskeleton, ruffle membrane, phagocytic cup.

UNIPROT

SwissProt: P55008 Human

SwissProt: O70200 Mouse

SwissProt: P55009 Rat

Synonyms

AIF 1 antibody

AIF-1 antibody

Aif1 antibody

AIF1 protein antibody

AIF1_HUMAN antibody

Allograft inflammatory factor 1 antibody

Allograft inflammatory factor 1 splice variant G antibody

allograft inflammatory factor-1 splice variant Hara-1 antibody

balloon angioplasty responsive transcription antibody

BART 1 antibody

Expand

Images

  • Immunocytochemistry analysis of RAW264.7 cells labeling Iba1 with Rabbit anti-Iba1 antibody (<a href="/products/HA720158F" style="font-weight: bold;text-decoration: underline;">HA720158F</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody (<a href="/products/HA720158F" style="font-weight: bold;text-decoration: underline;">HA720158F</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of RAW264.7 cells labeling Iba1 with Rabbit anti-Iba1 antibody (HA720158F) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody (HA720158F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of J774A.1 cells labeling Iba1 with Rabbit anti-Iba1 antibody (<a href="/products/HA720158F" style="font-weight: bold;text-decoration: underline;">HA720158F</a>) at 1/250 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody (<a href="/products/HA720158F" style="font-weight: bold;text-decoration: underline;">HA720158F</a>) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of J774A.1 cells labeling Iba1 with Rabbit anti-Iba1 antibody (HA720158F) at 1/250 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody (HA720158F) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of SH-SY5Y cells labeling Iba1.<br /><br />Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Iba1 (<a href="/products/HA720158F" style="font-weight: bold;text-decoration: underline;">HA720158F</a>, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor&trade; 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of SH-SY5Y cells labeling Iba1.

    Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Iba1 (HA720158F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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