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Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [SJ19-09]

Usd: 385

Specification

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Catalog# ET1606-49

Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [SJ19-09]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [SJ19-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Integrin beta 3 aa 729-788 / 788.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 87 kDa

Positive Control

U-87 MG cell lysates, HepG2, human spleen tissue, mouse testis tissue, human liver carcinoma tissue, human breast carcinoma tissue, mouse colon tissue, U87-MG-si-NT cell lysate.

Conjugation

unconjugated

Clone Number

SJ19-09

RRID

AB_3069744

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC IF-Cell
human
Tested Tested
Tested Tested
Not Recommended
Not Recommended
mouse
Not Recommended
Tested Tested
 
 
rat
Not Recommended
Tested Tested
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:400-1:1,000

  • IF-Tissue

  • 1:200

Target

Function

Integrins are heterodimers composed of noncovalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.

Background References

1. Klausen C et al. Cell motility and spreading are suppressed by HOXA4 in ovarian cancer cells: possible involvement of beta1 integrin. Mol Cancer Res 7:1425-37 (2009).

2. Liu, J. et al. The dual structural roles of the membrane distal region of the α-integrin cytoplasmic tail during integrin inside-out activation. Journal of cell science 128: 1718-31(2015).

Sequence Similarity

Belongs to the integrin beta chain family.

Tissue Specificity

Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.

Post-translational Modification

Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.

Subcellular Location

Cell membrane, Cell projection, Cell junction.

UNIPROT

SwissProt: P05106 Human

SwissProt: O54890 Mouse

Entrez Gene: 29302 Rat

Synonyms

BDPLT16 antibody

BDPLT2 antibody

CD 61 antibody

CD61 antibody

CD61 antigen antibody

GP3A antibody

GPIIIa antibody

GT antibody

HPA 1 antibody

HPA 4 antibody

Expand

Images

  • Western blot analysis of Integrin beta 3 on U-87 MG cell lysates with Rabbit anti-Integrin beta 3 antibody (<a href="/products/ET1606-49" style="font-weight: bold;text-decoration: underline;">ET1606-49</a>) at 1/2,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 87 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 1 minute 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1606-49" style="font-weight: bold;text-decoration: underline;">ET1606-49</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Integrin beta 3 on U-87 MG cell lysates with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/2,000 dilution.

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 87 kDa
    Observed band size: 100 kDa

    Exposure time: 1 minute 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-49) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody (<a href="/products/ET1606-49" style="font-weight: bold;text-decoration: underline;">ET1606-49</a>) at 1/500 dilution.<br /><br />Lane 1: U87-MG-si NT cell lysate<br />Lane 2: U87-MG-si Integrin beta 3 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 87 kDa<br />Observed band size: 87,100 kDa<br /><br />Exposure time: 1 minutes 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br /><a href="/products/ET1606-49" style="font-weight: bold;text-decoration: underline;">ET1606-49</a> was shown to specifically react with Integrin beta 3 in U87-MG-si NT cells. Weakened band was observed when U87-MG-si Integrin beta 3 sample was tested. U87-MG-si NT and U87-MG-si Integrin beta 3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1606-49" style="font-weight: bold;text-decoration: underline;">ET1606-49</a>, 1/500) and Loading control antibody (Rabbit anti-Hsp90, <a href="/products/ET1605-56" style="font-weight: bold;text-decoration: underline;">ET1605-56</a>, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/500 dilution.

    Lane 1: U87-MG-si NT cell lysate
    Lane 2: U87-MG-si Integrin beta 3 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 87 kDa
    Observed band size: 87,100 kDa

    Exposure time: 1 minutes 10 seconds;

    4-20% SDS-PAGE gel.

    ET1606-49 was shown to specifically react with Integrin beta 3 in U87-MG-si NT cells. Weakened band was observed when U87-MG-si Integrin beta 3 sample was tested. U87-MG-si NT and U87-MG-si Integrin beta 3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-49, 1/500) and Loading control antibody (Rabbit anti-Hsp90, ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Integrin beta 3 antibody (<a href="/products/ET1606-49" style="font-weight: bold;text-decoration: underline;">ET1606-49</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1606-49" style="font-weight: bold;text-decoration: underline;">ET1606-49</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-49) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Integrin beta 3 antibody (<a href="/products/ET1606-49" style="font-weight: bold;text-decoration: underline;">ET1606-49</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1606-49" style="font-weight: bold;text-decoration: underline;">ET1606-49</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-49) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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