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Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [PSH09-27]

Usd: 385

Specification

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Catalog# HA723085

Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [PSH09-27]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [PSH09-27]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Integrin beta 3 aa 101-150.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 87 kDa

Positive Control

U-87 MG cell lysate, LNCaP cell lysate, HUVEC cell lysate, C2C12 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, human kidney tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue.

Conjugation

unconjugated

Clone Number

PSH09-27

Reactivity Data

WB IHC-P IF-Tissue
Human
Tested Tested
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:2,000

  • IF-Tissue

  • 1:1,000

Target

Function

Integrin beta-3 (β3) or CD61 is a protein that in humans is encoded by the ITGB3 gene. CD61 is a cluster of differentiation found on thrombocytes. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface-mediated signaling. Defectively expressed β3 integrin subunit has been correlated with presence of endometriosis, and has been suggested as a putative marker of this condition.

Background References

1. Zhang Y et al. Integrin beta(3) directly inhibits the Galpha(13)-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis. Nat Commun. 2023 Aug

2. Hu HJ et al. Integrin beta 3-overexpressing mesenchymal stromal cells display enhanced homing and can reduce atherosclerotic plaque. World J Stem Cells. 2023 Sep

Subcellular Location

Cell membrane, Cell projection, lamellipodium membrane, Cell junction, focal adhesion, Postsynaptic cell membrane, Synapse.

UNIPROT

SwissProt: P05106 Human

SwissProt: O54890 Mouse

Entrez Gene: 29302 Rat

Synonyms

BDPLT16 antibody

BDPLT2 antibody

CD 61 antibody

CD61 antibody

CD61 antigen antibody

GP3A antibody

GPIIIa antibody

GT antibody

HPA 1 antibody

HPA 4 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody (<a href="/products/HA723085" style="font-weight: bold;text-decoration: underline;">HA723085</a>) at 1/5,000 dilution.<br /><br />Lane 1: U-87 MG cell lysate (20 µg/Lane)<br />Lane 2: LNCaP cell lysate (low expression) (20 µg/Lane)<br />Lane 3: HUVEC cell lysate (20 µg/Lane)<br />Lane 4: C2C12 cell lysate (20 µg/Lane)<br />Lane 5: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 6: Rat spleen tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 87 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723085" style="font-weight: bold;text-decoration: underline;">HA723085</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody (HA723085) at 1/5,000 dilution.

    Lane 1: U-87 MG cell lysate (20 µg/Lane)
    Lane 2: LNCaP cell lysate (low expression) (20 µg/Lane)
    Lane 3: HUVEC cell lysate (20 µg/Lane)
    Lane 4: C2C12 cell lysate (20 µg/Lane)
    Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 6: Rat spleen tissue lysate (40 µg/Lane)

    Predicted band size: 87 kDa
    Observed band size: 100 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723085) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: spleen<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Human

    Site: spleen

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Integrin beta 3 antibody (<a href="/products/HA723085" style="font-weight: bold;text-decoration: underline;">HA723085</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723085" style="font-weight: bold;text-decoration: underline;">HA723085</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Integrin beta 3 antibody (HA723085) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723085) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Integrin beta 3 antibody (<a href="/products/HA723085" style="font-weight: bold;text-decoration: underline;">HA723085</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723085" style="font-weight: bold;text-decoration: underline;">HA723085</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Integrin beta 3 antibody (HA723085) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723085) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Integrin beta 3 antibody (<a href="/products/HA723085" style="font-weight: bold;text-decoration: underline;">HA723085</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723085" style="font-weight: bold;text-decoration: underline;">HA723085</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Integrin beta 3 antibody (HA723085) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723085) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Integrin beta 3 antibody (<a href="/products/HA723085" style="font-weight: bold;text-decoration: underline;">HA723085</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723085" style="font-weight: bold;text-decoration: underline;">HA723085</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Integrin beta 3 antibody (HA723085) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723085) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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