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KDM5B Recombinant Rabbit Monoclonal Antibody [PSH03-97]

Usd: 385

Specification

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Catalog# HA722076

KDM5B Recombinant Rabbit Monoclonal Antibody [PSH03-97]

Application

  • WB

  • IP

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

KDM5B Recombinant Rabbit Monoclonal Antibody [PSH03-97]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human KDM5B aa 960-1,009 / 1,544.

Species Reactivity

Human

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 176 kDa

Positive Control

BT-20 cell lysate, MDA-MB-468 cell lysate, AGS cell lysate, A549 cell lysate.

Conjugation

unconjugated

Clone Number

PSH03-97

RRID

AB_3096793

Reactivity Data

WB IP
Human
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

PLU-1 is a large (1,544 amino acid) nuclear protein that interacts with brain factor-1 (BF-1) and paired box 9 (PAX9), both of which are developmental transcription factors. PLU-1 belongs to the testis-cancer antigen group of proteins and is a member of the ARID family of DNA binding proteins. It is a multi-domain protein with strong transcriptional repression properties. PLU-1 shows restricted expression in adult tissues, with high expression in testis and transient expression in the pregnant mammary gland. Both the PLU-1 gene and the PLU-1 protein product are specifically upregulated in breast cancer. PLU-1 may be important in meiotic transcription because of its apparent association with chromatin.

Background References

1. Tan K et al. Human PLU-1 has transcriptional repression properties and interacts with the developmental transcription factors BF-1 and PAX9. J Biol Chem 278:20507-20513 (2003).

2. Roesch A et al. Re-expression of the retinoblastoma-binding protein 2-homolog 1 reveals tumor-suppressive functions in highly metastatic melanoma cells. J Invest Dermatol 126:1850-1859 (2006).

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q9UGL1 Human

Synonyms

Cancer/testis antigen 31 antibody

CT31 antibody

Histone demethylase JARID1B antibody

JARID1B antibody

Jumonji AT rich interactive domain 1B antibody

Jumonji/ARID domain-containing protein 1B antibody

Kdm5b antibody

KDM5B_HUMAN antibody

Lysine (K) specific demethylase 5B antibody

Lysine demethylase 5B antibody

Expand

Images

  • Western blot analysis of KDM5B on different lysates with Rabbit anti-KDM5B antibody (<a href="/products/HA722076" style="font-weight: bold;text-decoration: underline;">HA722076</a>) at 1/2,000 dilution.<br /><br />Lane 1: BT-20 cell lysate<br />Lane 2: MDA-MB-468 cell lysate<br />Lane 3: AGS cell lysate<br />Lane 4: A549 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 176 kDa<br />Observed band size: 145 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722076" style="font-weight: bold;text-decoration: underline;">HA722076</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of KDM5B on different lysates with Rabbit anti-KDM5B antibody (HA722076) at 1/2,000 dilution.

    Lane 1: BT-20 cell lysate
    Lane 2: MDA-MB-468 cell lysate
    Lane 3: AGS cell lysate
    Lane 4: A549 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 176 kDa
    Observed band size: 145 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722076) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.

  • KDM5B was immunoprecipitated in 0.2mg A549 cell lysate with <a href="/products/HA722076" style="font-weight: bold;text-decoration: underline;">HA722076</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722076" style="font-weight: bold;text-decoration: underline;">HA722076</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: A549 cell lysate (input)<br />Lane 2: <a href="/products/ER1802-21" style="font-weight: bold;text-decoration: underline;">ER1802-21</a> IP in A549 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ER1802-21" style="font-weight: bold;text-decoration: underline;">ER1802-21</a> in A549 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 24 seconds

    KDM5B was immunoprecipitated in 0.2mg A549 cell lysate with HA722076 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722076 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: A549 cell lysate (input)
    Lane 2: ER1802-21 IP in A549 cell lysate
    Lane 3: Rabbit IgG instead of ER1802-21 in A549 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 24 seconds

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"