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KIAA0100 Recombinant Mouse Monoclonal Antibody [A0-F3-R]

Usd: 350

Specification

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Catalog# HA601317

KIAA0100 Recombinant Mouse Monoclonal Antibody [A0-F3-R]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

KIAA0100 Recombinant Mouse Monoclonal Antibody [A0-F3-R]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human KIAA0100 aa 1,947-2,235 / 2,235.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 254 kDa

Positive Control

MDA-MB-231 cell lysate, SK-Br-3 cell lysate, MCF7 cell lysate, HeLa cell lysate, A431 cell lysate, PC-3M cell lysate, LNCaP cell lysate, HeLa, human breast cancer tissue, human kidney tissue, human pancreas tissue.

Conjugation

unconjugated

Clone Number

A0-F3-R

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:2,000

Target

Function

Breast cancer-overexpressed gene 1 protein (KIAA0100) is a glucoprotein composed of 2,235 amino acids. KIAA0100 has four isforms (254kDa,250kDa,45kDa,25kDa).KIAA0100 is expressed in pancreas, placenta and up-regulated in breast carcinoma epithelial cells. This gene was initially characterized in human as having high expression levels in breast carcinomas and breast cancer cell lines. This gene also has increased expression in prostrate cancer cells relative to normal prostrate tissues. Expression of this gene is negatively regulated by direct binding of the microRNA miR-195 to its 3' UTR. miR-195 has been shown to modulate the invasiveness of prostrate cancer cells and xenograft metastases by downgrading expression of this gene. In mouse, the protein encoded by this gene was identified as an antigen on acute monocytic leukemia cells. In human, alternative splicing results in multiple transcript variants encoding distinct isoforms; some of these isoforms are predicted to contain an RNA pol II promoter FMP27 protein domain and a Golgi-body-localization APT1 domain.

Background References

1. "A quantitative atlas of mitotic phosphorylation."Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008)

2. "Serological identification of immunogenic antigens in acute monocytic leukemia."Chen G., Zhang W.G., Cao X.M., Li F.Y., Liu X.P., Yao L.B.Leuk. Res. 29:503-509(2005)

Subcellular Location

Cell membrane, Endoplasmic reticulum membrane, Mitochondrion membrane.

UNIPROT

SwissProt: Q14667 Human

Synonyms

Antigen MLAA-22 antibody

BCOX antibody

BCOX1 antibody

Breast cancer-overexpressed gene 1 protein antibody

CT101 antibody

DKFZp686M0843 antibody

K0100_HUMAN antibody

KIAA0100 antibody

MGC111488 antibody

MGC134981 antibody

Expand

Images

  • Western blot analysis of KIAA0100 on different lysates with Mouse anti-KIAA0100 antibody (<a href="/products/HA601317" style="font-weight: bold;text-decoration: underline;">HA601317</a>) at 1/1,000 dilution.<br /><br />Lane 1: MDA-MB-231 cell lysate<br />Lane 2: SK-Br-3 cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: HeLa cell lysate<br />Lane 5: A431 cell lysate<br />Lane 6: PC-3M cell lysate<br />Lane 7: LNCaP cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 254 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601317" style="font-weight: bold;text-decoration: underline;">HA601317</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of KIAA0100 on different lysates with Mouse anti-KIAA0100 antibody (HA601317) at 1/1,000 dilution.

    Lane 1: MDA-MB-231 cell lysate
    Lane 2: SK-Br-3 cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: HeLa cell lysate
    Lane 5: A431 cell lysate
    Lane 6: PC-3M cell lysate
    Lane 7: LNCaP cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 254 kDa
    Observed band size: 55 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601317) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling KIAA0100 with Mouse anti-KIAA0100 antibody (<a href="/products/HA601317" style="font-weight: bold;text-decoration: underline;">HA601317</a>) at 1/100 dilution.<br /><br />Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-KIAA0100 antibody (<a href="/products/HA601317" style="font-weight: bold;text-decoration: underline;">HA601317</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of HeLa cells labeling KIAA0100 with Mouse anti-KIAA0100 antibody (HA601317) at 1/100 dilution.

    Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-KIAA0100 antibody (HA601317) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-KIAA0100 antibody (<a href="/products/HA601317" style="font-weight: bold;text-decoration: underline;">HA601317</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601317" style="font-weight: bold;text-decoration: underline;">HA601317</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-KIAA0100 antibody (HA601317) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601317) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-KIAA0100 antibody (<a href="/products/HA601317" style="font-weight: bold;text-decoration: underline;">HA601317</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601317" style="font-weight: bold;text-decoration: underline;">HA601317</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-KIAA0100 antibody (HA601317) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601317) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-KIAA0100 antibody (<a href="/products/HA601317" style="font-weight: bold;text-decoration: underline;">HA601317</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601317" style="font-weight: bold;text-decoration: underline;">HA601317</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-KIAA0100 antibody (HA601317) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601317) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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