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L1CAM Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# 0805-5

L1CAM Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

L1CAM Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Human L1CAM aa 1,208-1,257 / 1,257.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 140 kDa

Positive Control

A375 cell lysate, HeLa cell lysate, human brain tissue lysate, rat brain tissue lysate, mouse brain tissue lysate, A375, 293T, Hela, human breast cancer tissue, human kidney tissue, mouse kidney tissue, SHG-44.

Conjugation

unconjugated

RRID

AB_3068660

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:600

  • FC

  • 1:50-1:100

Target

Function

The neural cell adhesion molecule L1 (NCAM-L1) is an immunoglobulin superfamily adhesion molecule that is critical for neural development, involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Defects in L1CAM are the cause of hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS). Hydrocephalus is a condition in which abnormal accumulation of cerebrospinal fluid in the brain causes increased intracranial pressure inside the skull. Defects in L1CAM are also the cause of mental retardation, aphasia, shuffling gait, and adducted thumbs syndrome (MASA), a X-linked disorder.

Background References

1. Fu TG et al. miR-143 inhibits oncogenic traits by degrading NUAK2 in glioblastoma. Int J Mol Med 37:1627-35 (2016).

2. Yoo M et al. Analysis of human embryonic stem cells with regulatable expression of the cell adhesion molecule l1 in regeneration after spinal cord injury. J Neurotrauma 31:553-64 (2014).

Sequence Similarity

Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.

Subcellular Location

Cell membrane, Cell projection, Membrane.

UNIPROT

SwissProt: P32004 Human

SwissProt: P11627 Mouse

SwissProt: Q05695 Rat

Synonyms

Antigen identified by monoclonal antibody

R1 antibody

CAML1 antibody

CD171 antibody

CD171 antigen antibody

HSAS antibody

HSAS1 antibody

Hyd antibody

L1 antibody

L1 cell adhesion molecule antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of L1CAM on different lysates with Rabbit anti-L1CAM antibody (<a href="/products/0805-5" style="font-weight: bold;text-decoration: underline;">0805-5</a>) at 1/1,000 dilution.<br /><br />Lane 1: A375 cell lysate<br />Lane 2: A549 cell lysate (low expression)<br />Lane 3: HeLa cell lysate<br />Lane 4: Human brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 140 kDa<br />Observed band size: 250 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/0805-5" style="font-weight: bold;text-decoration: underline;">0805-5</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of L1CAM on different lysates with Rabbit anti-L1CAM antibody (0805-5) at 1/1,000 dilution.

    Lane 1: A375 cell lysate
    Lane 2: A549 cell lysate (low expression)
    Lane 3: HeLa cell lysate
    Lane 4: Human brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 140 kDa
    Observed band size: 250 kDa

    Exposure time: 1 minute; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0805-5) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of A375 cells labeling L1CAM with Rabbit anti-L1CAM antibody (<a href="/products/0805-5" style="font-weight: bold;text-decoration: underline;">0805-5</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-L1CAM antibody (<a href="/products/0805-5" style="font-weight: bold;text-decoration: underline;">0805-5</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of A375 cells labeling L1CAM with Rabbit anti-L1CAM antibody (0805-5) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-L1CAM antibody (0805-5) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • ICC staining NCAML1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining NCAML1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining NCAML1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining NCAML1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-L1CAM antibody (<a href="/products/0805-5" style="font-weight: bold;text-decoration: underline;">0805-5</a>) at 1/600 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/0805-5" style="font-weight: bold;text-decoration: underline;">0805-5</a>) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-L1CAM antibody (0805-5) at 1/600 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0805-5) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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