L1CAM Recombinant Rabbit Monoclonal Antibody [JM11-05] - BSA and Azide free
Catalog# HA751842
L1CAM Recombinant Rabbit Monoclonal Antibody [JM11-05] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
L1CAM Recombinant Rabbit Monoclonal Antibody [JM11-05] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human L1CAM aa 900-1135 / 1257.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 140 kDa
Positive Control
A375 cell lysate, HeLa cell lysate, human brain tissue lysate, human kidney tissue, human appendix tissue, human brain tissue, human colon cancer tissue, human prostate cancer tissue.
Conjugation
unconjugated
Clone Number
JM11-05
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors, including neuroblastomas, rhabdomyosarcomas, Wilm's tumors, Ewing's sarcomas and some primitive myeloid malignancies. The NCAM-L1 adhesion molecule (CD171) plays an important role in axon guidance and cell migration in the nervous system. The presence of NCAM-L1 might contribute to tumor progression by promoting cell adhesion and migration and is known to be expressed by neurons, neuroblastomas and other malignant tumors.
Background References
1. Fu TG et al. miR-143 inhibits oncogenic traits by degrading NUAK2 in glioblastoma. Int J Mol Med 37:1627-35 (2016).
2. Yoo M et al. Analysis of human embryonic stem cells with regulatable expression of the cell adhesion molecule l1 in regeneration after spinal cord injury. J Neurotrauma 31:553-64 (2014).
Sequence Similarity
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Subcellular Location
Cell membrane, Cell projection, growth cone, axon, dendrite.
UNIPROT
Synonyms
Antigen identified by monoclonal antibody
R1 antibody
CAML1 antibody
CD171 antibody
CD171 antigen antibody
HSAS antibody
HSAS1 antibody
Hyd antibody
L1 antibody
L1 cell adhesion molecule antibody
ExpandImages
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☑ Relative expression (RE)
This data was developed using ET1703-51, the same antibody clone in a different buffer formulation.
Western blot analysis of L1CAM on different lysates with Rabbit anti-L1CAM antibody (ET1703-51) at 1/1,000 dilution.
Lane 1: A375 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (low expression) (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (20 µg/Lane)
Predicted band size: 140 kDa
Observed band size: 250 kDa
Exposure time: 10 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-51) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1703-51, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1703-51, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1703-51, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1703-51, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1703-51, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using ET1703-51, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
L1CAM Recombinant Rabbit Monoclonal Antibody [JM11-05]
Application: WB,IHC-P,IF-Tissue
Reactivity: Human
Conjugate: unconjugated
