iFluor™ 488 Conjugated Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07]
Catalog# HA720171F
iFluor™ 488 Conjugated Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07]
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IF-Tissue
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Human
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Mouse
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Rat
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Lamin B1 aa 511-560 / 586.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IF-Tissue
Molecular Weight
Predicted band size: 66 kDa
Positive Control
Human colon carcinoma tissue, mouse colon tissue, rat colon tissue.
Conjugation
iFluor™ 488
Clone Number
SI17-07
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:200
Target
Function
A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, functions as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.
Background References
1. Cruz OH et al. Multinucleation and Polykaryon Formation is Promoted by the EhPC4 Transcription Factor in Entamoeba histolytica. Sci Rep 6:19611 (2016).
2. Lund K et al. Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment. PLoS One 10:e0123684 (2015).
Sequence Similarity
Belongs to the intermediate filament family.
Post-translational Modification
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Subcellular Location
Nucleus inner membrane.
Synonyms
ADLD antibody
lamin B1 antibody
Lamin-B1 antibody
LMN antibody
LMN2 antibody
LMNB antibody
Lmnb1 antibody
LMNB1_HUMAN antibody
MGC111419 antibody
OTTHUMP00000159218 antibody
Images
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Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Lamin B1 with Rabbit anti-Lamin B1 antibody (HA720171F) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720171F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Lamin B1 with Rabbit anti-Lamin B1 antibody (HA720171F) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720171F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded rat colon tissue labeling Lamin B1 with Rabbit anti-Lamin B1 antibody (HA720171F) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720171F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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