14-3-3 alpha+beta Mouse Monoclonal Antibody [9-26]
Catalog# M0407-14
14-3-3 alpha+beta Mouse Monoclonal Antibody [9-26]
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Zebrafish
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
14-3-3 alpha+beta Mouse Monoclonal Antibody [9-26]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human 14-3-3 b / a aa 1-246 / 246.
Species Reactivity
Human, Mouse, Zebrafish (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 28 kDa
Positive Control
A549 cell lysates, mouse liver tissue lysates, hybrid fish (crucian-carp) brain tissue lysates, 293T, human breast carcinoma tissue, Zebrafish tissue.
Conjugation
unconjugated
Clone Number
9-26
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:200
Target
Function
The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints. The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol. The 14-3-3 proteins are also Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. They bind to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Background References
1. Carninci P., Kasukawa T., et al. Science 309:1559-1563(2005).
2. Sacksteder C.A., Qian W.-J.,et al. Biochemistry 45:8009-8022(2006).
3. Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617–47 (2000).
Sequence Similarity
Belongs to the 14-3-3 family.
Post-translational Modification
The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Subcellular Location
Cytoplasm, Membrane, Vacuole.
UNIPROT
Synonyms
14 3 3 alpha antibody
14 3 3 protein beta/alpha antibody
14-3-3 protein beta/alpha antibody
1433B_HUMAN antibody
Brain protein 14 3 3 beta isoform antibody
GW128 antibody
HS 1 antibody
KCIP-1 antibody
KCIP1 antibody
N-terminally processed antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of 14-3-3 alpha+beta on different lysates with Mouse anti-14-3-3 alpha+beta antibody (M0407-14) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-14-3-3 alpha+beta KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0407-14) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of 14-3-3 alpha+beta on A549 cell lysates with Mouse anti-14-3-3 alpha+beta antibody (M0407-14) at 1/5,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0407-14) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis on mouse liver tissue lysates using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).
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Western blot analysis of 14-3-3b/a on hybrid fish (crucian-carp) brain tissue lysate using anti-14-3-3b/a antibody at 1/500 dilution.
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Immunocytochemistry analysis of 293T cells labeling 14-3-3 alpha+beta with Mouse anti-14-3-3 alpha+beta antibody (M0407-14) at 1/50 dilution.
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Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).
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Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-14-3-3b/a antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
14-3-3 alpha+beta Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
