Junctophilin-2 Mouse Monoclonal Antibody [3-2]
Catalog# M0407-20
Junctophilin-2 Mouse Monoclonal Antibody [3-2]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Junctophilin-2 Mouse Monoclonal Antibody [3-2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human Junctophilin-2 aa 1-50 / 696.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 74 kDa
Positive Control
Rat skeletal muscle tissue lysates, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.
Conjugation
unconjugated
Clone Number
3-2
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
Junctophilin-2 (JPH2) is a cardiac specific member of the junctophilins, a newly characterized family of junctional membrane complex proteins important in physically approximating the plasmalemmal L-type calcium channel and the sarcoplasmic reticulum ryanodine receptor for calcium-induced calcium release. It acts probably by anchoring the plasma membrane and endoplasmic reticulum. JPH2 contributes to the construction of skeletal muscle triad junctions, and plays an essential role in heart development.
Background References
1. Takeshima, H., et al. 2000. Junctophilins: a novel family of junctional membrane complex proteins. Mol. Cell 6: 11-22.
2. Nishi, M., et al. 2003. Coexpression of Junctophilin type 3 and type 4 in brain. Brain Res. Mol. Brain Res. 118: 102-110.
Sequence Similarity
Belongs to the junctophilin family.
Tissue Specificity
Specifically expressed in skeletal muscle and heart.
Post-translational Modification
Phosphorylation on Ser-165, probably by PKC, affects RYR1-mediated calcium ion release, interaction with TRPC3, and skeletal muscle myotubule development.; Proteolytically cleaved by calpain in response to cardiac stress. The major cleavage site takes place at the C-terminus and leads to the release of the Junctophilin-2 N-terminal fragment chain (JP2NT).
Subcellular Location
Cell membrane.
Synonyms
FLJ40969 antibody
JP-2 antibody
JP2 antibody
Jph2 antibody
JPH2_HUMAN antibody
Junctophilin 2 antibody
Junctophilin type 2 antibody
Junctophilin-2 antibody
OTTHUMP00000031651 antibody
OTTHUMP00000031652 antibody
Images
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Western blot analysis of Junctophilin-2 on rat skeletal muscle tissue lysates with Mouse anti-Junctophilin-2 antibody (M0407-20) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0407-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Mouse anti-Junctophilin-2 antibody (M0407-20) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0407-20) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Mouse anti-Junctophilin-2 antibody (M0407-20) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0407-20) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Mouse anti-Junctophilin-2 antibody (M0407-20) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0407-20) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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