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Synaptophysin Mouse Monoclonal Antibody [A1-7]

Usd: 350

Specification

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Catalog# M0407-30

Synaptophysin Mouse Monoclonal Antibody [A1-7]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Synaptophysin Mouse Monoclonal Antibody [A1-7]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide (KLH-coupled) within human Synaptophysin 220-280 aa.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

34kDa

Positive Control

Mouse brain tissue lysates, PANC-1,rat pancreas tissue, medullary thyroid carcinoma tissue, human pancreas tissue, mouse brain tissue, SHG-44.

Conjugation

unconjugated

Clone Number

A1-7

RRID

AB_3072983

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:200-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms.

Background References

1. Janz R et al. Essential roles in synaptic plasticity for synaptogyrin I and synaptophysin I. Neuron 24:687-700 (1999).

2. Pekkurnaz G et al. Glucose regulates mitochondrial motility via Milton modification by O-GlcNAc transferase. Cell 158:54-68 (2014).

Sequence Similarity

Belongs to the synaptophysin/synaptobrevin family.

Tissue Specificity

Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.

Post-translational Modification

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

Subcellular Location

Cytoplasmic vesicle. Membrane. Synapse. Synaptosome.

UNIPROT

SwissProt: P08247 Human

SwissProt: Q62277 Mouse

SwissProt: P07825 Rat

Synonyms

Major synaptic vesicle protein p38 antibody

MRX96 antibody

MRXSYP antibody

Syn p38 antibody

Synaptophysin antibody

Syp antibody

SYPH antibody

SYPH_HUMAN antibody

SypI antibody

Images

  • Western blot analysis of Synaptophysin on mouse brain tissue lysate using anti-Synaptophysin antibody at 1/2,000 dilution.

    Western blot analysis of Synaptophysin on mouse brain tissue lysate using anti-Synaptophysin antibody at 1/2,000 dilution.

  • ICC staining Synaptophysin (red) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Synaptophysin (red) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-Synaptophysin antibody (<a href="/products/M0407-30" style="font-weight: bold;text-decoration: underline;">M0407-30</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M0407-30" style="font-weight: bold;text-decoration: underline;">M0407-30</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-Synaptophysin antibody (M0407-30) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0407-30) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded medullary thyroid carcinoma tissue with Mouse anti-Synaptophysin antibody (<a href="/products/M0407-30" style="font-weight: bold;text-decoration: underline;">M0407-30</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M0407-30" style="font-weight: bold;text-decoration: underline;">M0407-30</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded medullary thyroid carcinoma tissue with Mouse anti-Synaptophysin antibody (M0407-30) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0407-30) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Synaptophysin antibody (<a href="/products/M0407-30" style="font-weight: bold;text-decoration: underline;">M0407-30</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M0407-30" style="font-weight: bold;text-decoration: underline;">M0407-30</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Synaptophysin antibody (M0407-30) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0407-30) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Synaptophysin antibody (<a href="/products/M0407-30" style="font-weight: bold;text-decoration: underline;">M0407-30</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M0407-30" style="font-weight: bold;text-decoration: underline;">M0407-30</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Synaptophysin antibody (M0407-30) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0407-30) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"