DPPA2 Mouse Monoclonal Antibody [D0-G11]
Catalog# M0731-6
DPPA2 Mouse Monoclonal Antibody [D0-G11]
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WB
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Mouse
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unconjugated
Overview
Product Name
DPPA2 Mouse Monoclonal Antibody [D0-G11]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within mouse DPPA2 aa 1-50 / 301.
Species Reactivity
Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 34 kDa
Positive Control
F9 cell lysates.
Conjugation
unconjugated
Clone Number
D0-G11
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
Target
Function
DPPA2 (developmental pluripotency associated 2) is an embryo-cancer antigen that is coexpressed with cancer-testis antigens in non-small cell lung cancer. Dppa2 is closely linked SAP motif genes restricted to pluripotent cells and the germ line. It may play a role in maintaining cell pluripotentiality and in embryo preimplantation development.
Background References
1. Bortvin A., Eggan K., Skaletsky H., etc. "Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei."; Development 130:1673-1680(2003).
2. Du J., Lin G., Nie Z.Y., Lu G.X.; "Molecular cloning and characterization analysis of HPESCRG1, a novel gene expressed specifically in human embryonic stem cell.";Zhonghua Yi Xue Yi Chuan Xue Za Zhi 21:542-547(2004).
3. Carninci P., Kasukawa T., Katayama S., etc. Kawai J."The transcriptional landscape of the mammalian genome.";Science 309:1559-1563(2005).
Tissue Specificity
Not detected in adult tissues.
Subcellular Location
Nucleus
UNIPROT
Synonyms
cancer/testis antigen 100 antibody
CT100 antibody
Developmental Pluripotency Associated 2 antibody
developmental pluripotency-associated gene 2 antibody
developmental pluripotency-associated protein 2 antibody
ECAT15-2 antibody
embryonic stem cell (ESC) associated transcript 15-2 antibody
PESCRG1 antibody
Pluripotent embryonic stem cell related protein 1 antibody
Pluripotent embryonic stem cell-related gene 1 antibody
ExpandImages
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Western blot analysis of DPPA2 on F9 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0731-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"