Human Iambda light chain Mouse Monoclonal Antibody [36-59]
Catalog# M0806-2
Human Iambda light chain Mouse Monoclonal Antibody [36-59]
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WB
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ELISA
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Human
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unconjugated
Overview
Product Name
Human Iambda light chain Mouse Monoclonal Antibody [36-59]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human Iambda light chain aa 1-106/106.
Species Reactivity
Human
Validated Applications
WB, ELISA
Molecular Weight
25 kDa
Positive Control
Human IgG protein lysates.
Conjugation
unconjugated
Clone Number
36-59
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1000
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ELISA
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Use at an assay dependent concentration.
Target
Function
Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
Background References
1. Teng G.et.al.Immunoglobulin somatic hypermutation.Annu. Rev. Genet. 41:107-120(2007).
Subcellular Location
Cell membrane, Immunoglobulin, Membrane, Secreted
UNIPROT
Synonyms
Bence Jones protein antibody
BJP antibody
Constant region of lambda light chains antibody
Ig lambda chain C regions antibody
ig lambda-6 chain C region antibody
IGLC 1 antibody
IGLC 2 antibody
IGLC 3 antibody
IGLC antibody
IGLC1 antibody
ExpandImages
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Western blot analysis of Human Iambda light chain on human IgG protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0806-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"