Human Serum Albumin Mouse Monoclonal Antibody [8-A3]
Catalog# M0809-4
Human Serum Albumin Mouse Monoclonal Antibody [8-A3]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
Human Serum Albumin Mouse Monoclonal Antibody [8-A3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Full length native protein (purified) corresponding to human serum albumin.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 69 kDa
Positive Control
Purified HSA protein, human liver tissue.
Conjugation
unconjugated
Clone Number
8-A3
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | |
|---|---|---|
| human |
|
|
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
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IHC-P
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1:5,000
Target
Function
HSA is the most common protein in serum. It is produced in the liver and the concentration in serum is 35-50 mg/ml. HSA consists of a single polypeptide chain of 584 aminoacids, stabilized by 17 disulfide bridges. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. HSA binds a wide variety of hydrophobic ligands including fatty acids, bilirubin, thyroxine and hemin.
Background References
1. MINGETTIPP,RUFFNER DE,KUANG wJ,et a1．Molecular strutture of the human albumin gene is revealed by nucleotide sequence within ql 1-22 of chromosome 4 [J]．J Biol Chem,1986,261:6747．6757
2. LAWN RM,ADEMAL J,BOCK SC,et a1．The sequence of human serum albumin cDNA and its expression in E．coli[J]．Nucleic Acids Res．1981,9(22):6103—6l14
3. SUGIO S,KASHIMA A,MOCHIZUK S,et a1．Crystal structure of human serum albumin at 2．5 A。resolution[J]．Protein Eng,1999,12:439．446
Sequence Similarity
Belongs to the ALB/AFP/VDB family.
Tissue Specificity
Plasma.
Post-translational Modification
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.; Glycated in diabetic patients.; Phosphorylated by FAM20C in the extracellular medium.; Acetylated on Lys-223 by acetylsalicylic acid.
Subcellular Location
Secreted.
UNIPROT
Synonyms
alb antibody
ALBU_HUMAN antibody
Albumin (32 AA) antibody
Albumin (AA 34) antibody
Albumin antibody
Analbuminemia antibody
Bisalbuminemia antibody
Cell growth inhibiting protein 42 antibody
DKFZp779N1935 antibody
Dysalbuminemic hyperthyroxinemia antibody
ExpandImages
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Western blot analysis on HSA protein using anti-HSA Mouse mAb (Cat. # M0809-4).
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Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Human Serum Albumin antibody (M0809-4) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0809-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Albumin Recombinant Rabbit Monoclonal Antibody [PSH24-72]
Application: WB,IF-Cell,IHC-P,IF-Tissue,FC(Intra)
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
