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Human Serum Albumin Mouse Monoclonal Antibody [8-A3]

Usd: 350

Specification

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Catalog# M0809-4

Human Serum Albumin Mouse Monoclonal Antibody [8-A3]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Human Serum Albumin Mouse Monoclonal Antibody [8-A3]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Full length native protein (purified) corresponding to human serum albumin.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 69 kDa

Positive Control

Purified HSA protein, human liver tissue.

Conjugation

unconjugated

Clone Number

8-A3

RRID

AB_3072998

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:5,000

Target

Function

HSA is the most common protein in serum. It is produced in the liver and the concentration in serum is 35-50 mg/ml. HSA consists of a single polypeptide chain of 584 aminoacids, stabilized by 17 disulfide bridges. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. HSA binds a wide variety of hydrophobic ligands including fatty acids, bilirubin, thyroxine and hemin.

Background References

1. MINGETTIPP,RUFFNER DE,KUANG wJ,et a1．Molecular strutture of the human albumin gene is revealed by nucleotide sequence within ql 1-22 of chromosome 4 [J]．J Biol Chem,1986,261:6747．6757

2. LAWN RM,ADEMAL J,BOCK SC,et a1．The sequence of human serum albumin cDNA and its expression in E．coli[J]．Nucleic Acids Res．1981,9(22):6103—6l14

3. SUGIO S,KASHIMA A,MOCHIZUK S,et a1．Crystal structure of human serum albumin at 2．5 A。resolution[J]．Protein Eng,1999,12:439．446

Sequence Similarity

Belongs to the ALB/AFP/VDB family.

Tissue Specificity

Plasma.

Post-translational Modification

Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.; Glycated in diabetic patients.; Phosphorylated by FAM20C in the extracellular medium.; Acetylated on Lys-223 by acetylsalicylic acid.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P02768 Human

Synonyms

alb antibody

ALBU_HUMAN antibody

Albumin (32 AA) antibody

Albumin (AA 34) antibody

Albumin antibody

Analbuminemia antibody

Bisalbuminemia antibody

Cell growth inhibiting protein 42 antibody

DKFZp779N1935 antibody

Dysalbuminemic hyperthyroxinemia antibody

Expand

Images

  • Western blot analysis on HSA protein using anti-HSA Mouse mAb (Cat. # <a href="/products/M0809-4" style="font-weight: bold;text-decoration: underline;">M0809-4</a>).

    Western blot analysis on HSA protein using anti-HSA Mouse mAb (Cat. # M0809-4).

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Human Serum Albumin antibody (<a href="/products/M0809-4" style="font-weight: bold;text-decoration: underline;">M0809-4</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M0809-4" style="font-weight: bold;text-decoration: underline;">M0809-4</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Human Serum Albumin antibody (M0809-4) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0809-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"