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6X His tag Mouse Monoclonal Antibody [8-F0]

Usd: 350

Specification

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Catalog# M0812-3

6X His tag Mouse Monoclonal Antibody [8-F0]

Application

  • WB

  • IP

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

6X His tag Mouse Monoclonal Antibody [8-F0]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide immune sequence is CHHHHHHHH.

Species Reactivity

Species independent

Validated Applications

WB, IP

Positive Control

C-terminal His-tagged recombinant protein.

Conjugation

unconjugated

Clone Number

8-F0

RRID

AB_3072999

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol.

Isotype

IgG2a

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IP

  • 2-5 µg/ml.

Target

Function

A variety of plasmids contain DNA that encodes a tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. This antibody can be used to detect the carboxyl-terminal 6×his tag in recombinant protein.

Background References

暂无

Synonyms

6 His epitope tag antibody

Hexa His tag antibody

HHHHHH epitope tag antibody

HHHHHH tag antibody

His tag antibody

Polyhistidine Tag antibody

Images

  • Western blot analysis of 6X His tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/M0812-3" style="font-weight: bold;text-decoration: underline;">M0812-3</a>, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: C-terminal His-tagged recombinant protein<br />Lane 2: N-terminal His-tagged recombinant protein

    Western blot analysis of 6X His tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0812-3, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: C-terminal His-tagged recombinant protein
    Lane 2: N-terminal His-tagged recombinant protein

  • His tag was immunoprecipitated in 2µg C terminal His Tag fusion protein lysate with <a href="/products/M0812-3" style="font-weight: bold;text-decoration: underline;">M0812-3</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/R1207-2" style="font-weight: bold;text-decoration: underline;">R1207-2</a> at 1/500 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: His Tag fusion protein lysate (input).<br />Lane 2: <a href="/products/M0812-3" style="font-weight: bold;text-decoration: underline;">M0812-3</a> IP in His Tag fusion protein lysate.<br />Lane 3: Mouse IgG instead of <a href="/products/M0812-3" style="font-weight: bold;text-decoration: underline;">M0812-3</a> in His Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    His tag was immunoprecipitated in 2µg C terminal His Tag fusion protein lysate with M0812-3 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1207-2 at 1/500 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.

    Lane 1: His Tag fusion protein lysate (input).
    Lane 2: M0812-3 IP in His Tag fusion protein lysate.
    Lane 3: Mouse IgG instead of M0812-3 in His Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation