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Human IgG kapa light chain Mouse Monoclonal Antibody [A11-G11]

Usd: 185

Specification

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Catalog# M0908-1

Human IgG kapa light chain Mouse Monoclonal Antibody [A11-G11]

Application

  • WB

  • ELISA

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Human IgG kapa light chain Mouse Monoclonal Antibody [A11-G11]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Native protein.

Species Reactivity

Human

Validated Applications

WB, ELISA

Molecular Weight

25 kDa

Positive Control

Human IgG protein lysates.

Conjugation

unconjugated

Clone Number

A11-G11

RRID

AB_3073001

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • ELISA

  • 1:5,000

Target

Function

IgG is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. This antibody reacts with kappa-light-chain of human IgG.

Background References

1. Teng G.et.al.Immunoglobulin somatic hypermutation.Annu. Rev. Genet. 41:107-120(2007).

Subcellular Location

Cell membrane, Immunoglobulin, Membrane, Secreted

UNIPROT

SwissProt: Q6P5S8 Human

Synonyms

Human IgG kapa light chain antibody

IGK@ protein antibody

IGK@ antibody

Images

  • Western blot analysis of Human IgG kapa light chain on human IgG protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/M0908-1" style="font-weight: bold;text-decoration: underline;">M0908-1</a>, 1/5000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Human IgG kapa light chain on human IgG protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0908-1, 1/5000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"