Skip to content

Goat IgG Light chain Mouse Monoclonal Antibody [B8-C4-G4]

Usd: 350

Specification

Bulk Order Quote | Customization Request

Catalog# M0910-2

Goat IgG Light chain Mouse Monoclonal Antibody [B8-C4-G4]

Application

  • WB

  • ELISA

  • IP

Reactivity

  • Goat

Conjugation

  • unconjugated

Overview

Product Name

Goat IgG Light chain Mouse Monoclonal Antibody [B8-C4-G4]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Native protein.

Species Reactivity

Goat

Validated Applications

WB, ELISA, IP

Molecular Weight

25 kDa

Positive Control

Goat IgG protein lysates.

Conjugation

unconjugated

Clone Number

B8-C4-G4

RRID

AB_3073003

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • ELISA

  • 1:2,000-1:5,000

  • IP

  • 1:100-1:500

Target

Function

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.TRITC(tetraethyl rhodamine isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. This monoclonal antibody can be used to detect the light chain of goat immunoglobulin.

Background References

暂无

Images

  • Western blot analysis of Goat IgG Light chain on Goat IgG protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/M0910-2" style="font-weight: bold;text-decoration: underline;">M0910-2</a>, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Goat IgG Light chain on Goat IgG protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"