Kidins220 Mouse Monoclonal Antibody [3-B6]
Catalog# M0910-4
Kidins220 Mouse Monoclonal Antibody [3-B6]
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WB
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IF-Cell
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IHC-P
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Mouse
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Human
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unconjugated
Overview
Product Name
Kidins220 Mouse Monoclonal Antibody [3-B6]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein with Human kinase D-interacting substrate 220 aa 1601-1771 / 1,771.
Species Reactivity
Mouse, Human
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 197kDa
Positive Control
K562 cell lysates, F9 cells, mouse brain tissue.
Conjugation
unconjugated
Clone Number
3-B6
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:10,000
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IF-Cell
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1:100
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IHC-P
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1:50
Target
Function
Kinase D-interacting substrate 220 (Kidins220), also known as ARMS (ankyrin-rich membrane spanning) is an integral plasma membrane protein predominantly expressed in brain and neurons that presents a polarized location in neural cells, concentrating at the tip of extending neurites and in plasma membrane micro-domains corresponding to lipid rafts. The primary amino acid sequence of Kidins220 reveals 11 ankyrin repeats, 4 trans-membrane domains, a proline-rich region, a sterile-α motif (or SAM) domain, and a type I postsynaptic density-95/discs large/zonula occludens-1 (PDZ)-binding motif at the very C-terminus.
Background References
1. " Identification and cloning of Kidins220, a novel neuronal substrate of protein kinase D.” Iglesias T, Cabrera-Poch N, Mitchell MP, Naven TJ, Rozengurt E, Schiavo G. J Biol Chem. 22;275(51):40048-56(2000).
2. "Large-scale phosphorylation analysis of mouse liver."Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007)
Subcellular Location
Cell membrane
Synonyms
Ankyrin repeat-rich membrane-spanning protein antibody
arms antibody
KDIS_HUMAN antibody
kidins220 antibody
Kinase D interacting substance of 220 kDa antibody
Kinase D-interacting substrate of 220 kDa antibody
rgd 619949 antibody
Images
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Western blot analysis of Kidins220 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Western blot analysis on recombinant protein using anti-Kidins220 Mouse mAb (Cat. # M0910-4).
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Immunofluorescent staining of F9 cells using anti-Kidins220 Mouse mAb (Cat. # M0910-4).
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Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-Kidins220 Mouse mAb (Cat. # M0910-4).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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