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GFP Mouse Monoclonal Antibody [B2-F7-C10]

Usd: 350

Specification

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Catalog# M1004-8

GFP Mouse Monoclonal Antibody [B2-F7-C10]

Application

  • WB

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

GFP Mouse Monoclonal Antibody [B2-F7-C10]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant full length protein of Aequorea victoria GFP.

Species Reactivity

Species independent

Validated Applications

WB

Molecular Weight

26 kDa

Positive Control

K562 cell lysates transfected with GFP.

Conjugation

unconjugated

Clone Number

B2-F7-C10

RRID

AB_3073013

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

Target

Function

The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.

Background References

1. Yu, H. et al. 2016. AAV-Mediated Gene Transfer to Dorsal Root Ganglion. Methods in molecular biology (Clifton, N.J.). 1382: 251-61.

2. Yamaoka, M. et al. 2016. PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a. J. Cell. Sci. 129: 637-49.

UNIPROT

SwissProt: P42212 AequoreaVictoria

Synonyms

GFP antibody

Green fluorescent protein antibody

Images

  • Western blot analysis of GFP on K562 cell lysates transfected with GFP. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/M1004-8" style="font-weight: bold;text-decoration: underline;">M1004-8</a>, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GFP on K562 cell lysates transfected with GFP. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1004-8, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation