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HRP Conjugated Mouse anti-Human IgG light chain(kappa chain) Mouse Monoclonal Antibody [A11-G11]

Usd: 374

Specification

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Catalog# M1007-11

HRP Conjugated Mouse anti-Human IgG light chain(kappa chain) Mouse Monoclonal Antibody [A11-G11]

Application

  • WB

  • ELISA

Reactivity

  • Human

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HRP Conjugated Mouse anti-Human IgG light chain(kappa chain) Mouse Monoclonal Antibody [A11-G11]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Human IgG light chain

Species Reactivity

Human

Validated Applications

WB, ELISA

Molecular Weight

25 kDa

Positive Control

Human IgG

Conjugation

HRP

Clone Number

A11-G11

RRID

AB_3073018

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.

Isotype

IgG2a

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • ELISA

  • 1:1,000

Target

Function

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Background References

暂无

Subcellular Location

Serum/ Plasma

Images

  • Representative standard curve using Mouse mAb (Cat. # <a href="/products/M1007-11" style="font-weight: bold;text-decoration: underline;">M1007-11</a>).

    Representative standard curve using Mouse mAb (Cat. # M1007-11).

  • Western blot analysis of Mouse anti-Human IgG light chain(kappa chain) on different lysates with Mouse anti-Human IgG light chain(kappa chain) antibody (<a href="/products/M1007-11" style="font-weight: bold;text-decoration: underline;">M1007-11</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human IgG kappa light chain<br />Lane 2: Rabbit IgG (negative)<br /><br />Lysates/proteins at 50 ng/Lane.<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1007-11" style="font-weight: bold;text-decoration: underline;">M1007-11</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of Mouse anti-Human IgG light chain(kappa chain) on different lysates with Mouse anti-Human IgG light chain(kappa chain) antibody (M1007-11) at 1/2,000 dilution.

    Lane 1: Human IgG kappa light chain
    Lane 2: Rabbit IgG (negative)

    Lysates/proteins at 50 ng/Lane.

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1007-11) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

  • Western blot analysis on human IgG κ chain using anti-Human IgG light chain(kappa chain) conjugated HRP Mouse mAb (Cat. # <a href="/products/M1007-11" style="font-weight: bold;text-decoration: underline;">M1007-11</a>).

    Western blot analysis on human IgG κ chain using anti-Human IgG light chain(kappa chain) conjugated HRP Mouse mAb (Cat. # M1007-11).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation