HRP Conjugated Mouse anti goat IgG lighty chain Mouse Monoclonal Antibody [B8-C4]
Catalog# M1007-14
HRP Conjugated Mouse anti goat IgG lighty chain Mouse Monoclonal Antibody [B8-C4]
-
WB
-
ELISA
-
Goat
-
HRP
Overview
Product Name
HRP Conjugated Mouse anti goat IgG lighty chain Mouse Monoclonal Antibody [B8-C4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Purified goat IgG lighty chain.
Species Reactivity
Goat
Validated Applications
WB, ELISA
Molecular Weight
Predicted band size: 25 kDa
Positive Control
Goat serum
Conjugation
HRP
Clone Number
B8-C4
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.
Isotype
IgG2b
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:5,000
-
ELISA
-
1:5,000
Target
Function
The fundamental structure of IgG contains two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa, thus tetrameric quaternary structure. Light chain, which is usually same in a natural IgG molecule, includesκchain andλchain, but κorλantibody can exist in one body simultaneously. A heavy chain includes Fab- and Fc-Fragments. This monoclonal antibody can be used to detect the light chain of goat immunoglobulin.
Background References
暂无
Subcellular Location
Secreted
Images
-
Western blot analysis of Mouse anti goat IgG lighty chain on Goat IgG lysate with Mouse anti-Mouse anti goat IgG lighty chain antibody (M1007-14) at 1/5,000 dilution.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1007-14) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"