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HSP60 Mouse Monoclonal Antibody [9-49]

Usd: 350

Specification

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Catalog# M1007-9

HSP60 Mouse Monoclonal Antibody [9-49]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HSP60 Mouse Monoclonal Antibody [9-49]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human HSP60 aa 301-350 / 573.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 61kDa

Positive Control

HeLa, HepG2, human kidney tissue, mouse kidney tissue, human lung cancerr tissue.

Conjugation

unconjugated

Clone Number

9-49

RRID

AB_3073023

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1

  • 1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:50-400

Target

Function

HSP60, also known as chaperonins (Cpn), is a family of heat shock proteins originally sorted by their 60kDa molecular mass. They prevent misfolding of proteins during stressful situations such as high heat, by assisting protein folding. HSP60 belong to a large class of molecules that assist protein folding, called molecular chaperones. Newly made proteins usually must fold from a linear chain of amino acids into a three-dimensional tertiary structure. The energy to fold proteins is supplied by non-covalent interactions between the amino acid side chains of each protein, and by solvent effects. Most proteins spontaneously fold into their most stable three-dimensional conformation, which is usually also their functional conformation, but occasionally proteins mis-fold. Molecular chaperones catalyze protein refolding by accelerating partial unfolding of misfolded proteins, aided by energy supplied by the hydrolysis of adenosine triphosphate (ATP). Chaperonin proteins may also tag misfolded proteins to be degraded.

Background References

1. Singh B., Patel H.V., Ridley R.G., Freeman K.B., Gupta R.S.; "Mitochondrial import of the human chaperonin (HSP60) protein."; Biochem. Biophys. Res. Commun. 169:391-396(1990).

2. Tanaka Y., Kanai F., Kawakami T., Tateishi K., Ijichi H., Kawabe T., Arakawa Y., Kawakami T., Nishimura T., Shirakata Y., Koike K., Omata M.; "Interaction of the hepatitis B virus X protein (HBx) with heat shock protein 60 enhances HBx-mediated apoptosis."; Biochem. Biophys. Res. Commun. 318:461-469(2004).

Sequence Similarity

Belongs to the chaperonin (HSP60) family.

Subcellular Location

Mitochondrion matrix

UNIPROT

SwissProt: P10809 Human

SwissProt: P63038 Mouse

SwissProt: P63039 Rat

Synonyms

60 kDa chaperonin antibody

60 kDa heat shock protein, mitochondrial antibody

CH60_HUMAN antibody

Chaperonin 60 antibody

Chaperonin, 60-KD antibody

CPN60 antibody

fa04a05 antibody

GROEL antibody

heat shock 60kDa protein 1 (chaperonin) antibody

Heat shock protein 1 (chaperonin) antibody

Expand

Images

  • Western blot analysis on Hela lysates using anti-HSP60 Mouse mAb (Cat. # <a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>).

    Western blot analysis on Hela lysates using anti-HSP60 Mouse mAb (Cat. # M1007-9).

  • Immunocytochemistry analysis of HeLa cells labeling HSP60 with Mouse anti-HSP60 antibody (<a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSP60 antibody (<a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling HSP60 with Mouse anti-HSP60 antibody (M1007-9) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSP60 antibody (M1007-9) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of HepG2 cells labeling HSP60 with Mouse anti-HSP60 antibody (<a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-HSP60 antibody (<a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of HepG2 cells labeling HSP60 with Mouse anti-HSP60 antibody (M1007-9) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-HSP60 antibody (M1007-9) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-HSP60 Mouse mAb (Cat. # <a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>).

    Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-HSP60 Mouse mAb (Cat. # M1007-9).

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-HSP60 antibody (<a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-HSP60 antibody (M1007-9) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-9) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-HSP60 antibody (<a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-HSP60 antibody (M1007-9) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-9) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung cancerr tissue with Mouse anti-HSP60 antibody (<a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1007-9" style="font-weight: bold;text-decoration: underline;">M1007-9</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung cancerr tissue with Mouse anti-HSP60 antibody (M1007-9) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-9) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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