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FAM62B Mouse Monoclonal Antibody [C4-D0]

Usd: 350

Specification

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Catalog# M1009-1

FAM62B Mouse Monoclonal Antibody [C4-D0]

Application

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

FAM62B Mouse Monoclonal Antibody [C4-D0]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human FAM62B aa 300-550.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 102 kDa

Positive Control

Human colon cancer tissue, human kidney tissue.

Conjugation

unconjugated

Clone Number

C4-D0

RRID

AB_3073028

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • IHC-P

  • 1:200

Target

Function

Tethers the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane. Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport. Plays a role in FGF signaling via its role in the rapid internalization of FGFR1 that has been activated by FGF1 binding; this occurs most likely via the AP-2 complex. Promotes the localization of SACM1L at endoplasmic reticulum-plasma membrane contact sites (EPCS).

Background References

1. Min S.-W et. al. E-Syts, a family of membranous Ca2+-sensor proteins with multiple C2 domains. Proc. Natl. Acad. Sci. U.S.A. 104:3823-3828(2007).

Subcellular Location

Cell membrane, Endoplasmic reticulum, Membrane.

UNIPROT

SwissProt: A0FGR8 Human

Synonyms

Chr2 synaptotagmin antibody

CHR2SYT antibody

E Syt2 antibody

ESYT 2 antibody

ESYT2 antibody

Extended synaptotagmin 2 antibody

Extended synaptotagmin like protein 2 antibody

FAM 62B antibody

Family with sequence similarity 62 (C2 domain containing) member B antibody

Family with sequence similarity 62 member B antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-FAM62B antibody (<a href="/products/M1009-1" style="font-weight: bold;text-decoration: underline;">M1009-1</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1009-1" style="font-weight: bold;text-decoration: underline;">M1009-1</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-FAM62B antibody (M1009-1) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1009-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-FAM62B antibody (<a href="/products/M1009-1" style="font-weight: bold;text-decoration: underline;">M1009-1</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1009-1" style="font-weight: bold;text-decoration: underline;">M1009-1</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-FAM62B antibody (M1009-1) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1009-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"