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LRRN3 Mouse Monoclonal Antibody [A4-6-3]

Usd: 350

Specification

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Catalog# M1012-8

LRRN3 Mouse Monoclonal Antibody [A4-6-3]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

LRRN3 Mouse Monoclonal Antibody [A4-6-3]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human LRRN3 aa 1-260.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

79 kDa

Positive Control

SHSY5Ycell lysate, rat testis tissue lysate, F9.

Conjugation

unconjugated

Clone Number

A4-6-3

RRID

AB_3073038

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgM

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:100

Target

Function

The LRRN3 is located on human chromosome 7, at 7q31.1.It contains 6 distinct gt-ag introns, and transcription produces five different mRNAs that appear to differ by truncation of the 3' end. There are only three main transcript variants that actually encode for the LRRN3 protein, with the longest transcript variant being 3744 base pairs in length. All three of these transcript variants have differing lengths and number of exons, but they all have the exon that includes the entire coding sequence for the LRRN3 protein.[8] There are also two paralogs to the LRRN3 gene. These include the LRRN1 and LRRN2 genes, both of which have the same leucine-rich repeats that are characteristic to this family of genes.

Background References

暂无

Subcellular Location

Membrane

UNIPROT

SwissProt: Q9H3W5 Human

SwissProt: Q8CBC6 Mouse

SwissProt: Q9ESY6 Rat

Synonyms

Leucine-rich repeat neuronal protein 3L antibidy

Neuronal leucine-rich repeat protein 3 antibidy

NLRR-3 antibidy

Nbla10363 antibidy

UNQ194 antibidy

LRRN3 antibidy

PRO220 antibidy

Images

  • Western blot analysis of LRRN3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/M1012-8" style="font-weight: bold;text-decoration: underline;">M1012-8</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: SHSY5Ycell lysate<br />Lane 2: Rat testis tissue lysate

    Western blot analysis of LRRN3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1012-8, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SHSY5Ycell lysate
    Lane 2: Rat testis tissue lysate

  • ICC staining of LRRN3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/M1012-8" style="font-weight: bold;text-decoration: underline;">M1012-8</a>, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of LRRN3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1012-8, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation