HRP Conjugated beta Actin Mouse Monoclonal Antibody [A2-F6]
Catalog# M1210-5
HRP Conjugated beta Actin Mouse Monoclonal Antibody [A2-F6]
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WB
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Human
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Mouse
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Rat
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HRP
Overview
Product Name
HRP Conjugated beta Actin Mouse Monoclonal Antibody [A2-F6]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within N-terminal human Beta-actin .
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
42 kDa
Positive Control
PC12 cell lysate, Hela cell lysate, NIH/3T3 cell lysate.
Conjugation
HRP
Clone Number
A2-F6
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
Target
Function
Beta actin also know as Cytoplasmic Actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error.
Background References
1. Fujiki R et al. GlcNAcylation of a histone methyltransferase in retinoic-acid-induced granulopoiesis. Nature 459:455-459 (2009).
Subcellular Location
Cytoplasm.
Synonyms
A26C1A antibody
A26C1B antibody
ACTB antibody
ACTB_HUMAN antibody
Actin beta antibody
Actin cytoplasmic 1 antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Actx antibody
b actin antibody
Beta cytoskeletal actin antibody
ExpandImages
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Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (M1210-5) at 1/5,000 dilution.
Lane 1: Mouse lung tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse colon tissue lysate
Lane 4: Rat lung tissue lysate
Lane 5: Rat spleen tissue lysate
Lane 6: Rat colon tissue lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: M1210-5, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Predicted band size: 42 kDa
Observed band size: 42 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis
Journal: Journal Of Advanced Research
DOI: 10.1016/j.jare.2026.02.060
IF: 13
Application: WB
Reactivity: Mouse
Publish date: 2026 Mar
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CXCL5 activates CXCR2 in nociceptive sensory neurons to drive joint pain and inflammation in experimental gouty arthritis
Journal: Nature Communications
DOI:
IF: 16.6
Application: WB
Reactivity: Mouse
Publish date: 2024 Apr
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Accelerating corneal wound healing using exosome-mediated targeting of NF-κB c-Rel
Journal: Inflammation And Regeneration
DOI:
IF: 10.426
Application: WB
Reactivity: Mouse
Publish date: 2023 Jan
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MYCN protein stability is a better prognostic indicator in neuroblastoma
Journal: BMC Pediatrics
DOI:
IF: 2.567
Application: WB
Reactivity: Human
Publish date: 2022 Jul
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A. Muciniphila Suppresses Colorectal Tumorigenesis by Inducing TLR2/NLRP3-Mediated M1-Like TAMs
Journal: Cancer Immunology Research
DOI:
IF: 10.1
Application: WB
Reactivity: Mouse
Publish date: 2021 Oct
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Shp2 in myocytes is essential for cardiovascular and neointima development.
Journal: Journal Of Molecular And Cellular Cardiology
DOI:
IF: 5.055
Application: WB
Reactivity: Mouse
Publish date: 2019 Dec
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mTOR-mediated glycolysis contributes to the enhanced suppressive function of murine tumor-infiltrating monocytic myeloid-derived suppressor cells
Journal: Cancer Immunology Immunotherapy
DOI: 10.1007/s00262-018-2177-1
IF: 4.225
Application: WB
Reactivity: Mouse
Publish date: 2018 Jul
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Tumor necrosis factor‑α‑induced protein‑8 like 2 regulates lipopolysaccharide‑induced rat rheumatoid arthritis immune responses and is associated with Rac activation and interferon regulatory factor 3 phosphorylation
Journal: Molecular Medicine Reports
DOI: 10.3892/mmr.2017.7311
IF: 1.692
Application: WB
Reactivity: Rat
Publish date: 2017 Aug
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