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TMEM177 Mouse Monoclonal Antibody [A6-A11-9]

Usd: 350

Specification

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Catalog# M1306-1

TMEM177 Mouse Monoclonal Antibody [A6-A11-9]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

TMEM177 Mouse Monoclonal Antibody [A6-A11-9]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human TMEM177 aa 1-311 / 311.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 34 kDa

Positive Control

TMEM177 recombinant protein, human liver tissue, HepG2, Hela, MCF-7.

Conjugation

unconjugated

Clone Number

A6-A11-9

RRID

AB_3073060

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:1,000

Target

Function

A transmembrane protein (TMEM) is a type of integral membrane protein that spans the entirety of the biological membrane to which it is permanently attached. Many transmembrane proteins function as gateways to permit the transport of specific substances across the biological membrane. They frequently undergo significant conformational changes to move a substance through the membrane. Transmembrane proteins are polytopic proteins that aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.

Background References

1. Isotta Lorenzi et al. The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis. Biochim Biophys Acta Mol Cell Res. 2018 Feb

Sequence Similarity

Belongs to the TMEM177 family.

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: Q53S58 Human

Synonyms

TM177_HUMAN antibody

Tmem177 antibody

Transmembrane protein 177 antibody

Images

  • Western blot analysis of TMEM177 on recombinant protein using anti-TMEM177 antibody at 1/1,000 dilution.

    Western blot analysis of TMEM177 on recombinant protein using anti-TMEM177 antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-TMEM177 antibody (<a href="/products/M1306-1" style="font-weight: bold;text-decoration: underline;">M1306-1</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1306-1" style="font-weight: bold;text-decoration: underline;">M1306-1</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-TMEM177 antibody (M1306-1) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1306-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ICC staining TMEM177 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TMEM177 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining TMEM177 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TMEM177 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining TMEM177 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TMEM177 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"