NusA Mouse Monoclonal Antibody [A6-E7]
Catalog# M1307-1
NusA Mouse Monoclonal Antibody [A6-E7]
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WB
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ELISA
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Escherichia coli
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unconjugated
Overview
Product Name
NusA Mouse Monoclonal Antibody [A6-E7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant full length protein of Escherichia coli NusA.
Species Reactivity
Escherichia coli
Validated Applications
WB, ELISA
Molecular Weight
Predicted band size: 55 kDa
Positive Control
E. coli lysates.
Conjugation
unconjugated
Clone Number
A6-E7
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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ELISA
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1:1,000-1:5,000
Target
Function
NusA is a key component in both prevention and enhancement of transcriptional termination. It is important in both Rho-dependent and intrinsic termination, as well as in lambda and other phage antitermination systems. The gene was first identified by isolation of the nusAl mutation, which restricts bacteriophage l growth by preventing the antitermination activity of the l N protein. NusA is involved in transcriptional antitermination in the cell. It has been shown to specifically aid in read-through of the RNA polymerase genes rpoB and rpoC, as well as in successful synthesis of the ribosomal RNA genes.
Background References
1. "Effect of NusA protein on expression of the nusA,infB operon in E. coli." Plumbridge J.A., Dondon J., Nakamura Y., Grunberg-Manago M. Nucleic Acids Res. 13:3371-3388(1985)
2. "Control of intrinsic transcription termination by N and NusA: the basic mechanisms."Gusarov I., Nudler E.Cell 107:437-449(2001)
Sequence Similarity
Belongs to the NusA family.
Subcellular Location
Cytoplasm.
Synonyms
Transcription termination/antitermination protein NusA
N utilization substance protein A
Transcription termination/antitermination L factor
nusA
b3169
JW3138
Images
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Western blot analysis of NusA on E. coli lysates with Mouse anti-NusA antibody (M1307-1) at 1/500 dilution.
Lane 1: E. coli lysate (2 µL/Lane)
Lane 2: E. coli lysate (5 µL/Lane)
Lane 3: E. coli lysate (10 µL/Lane)
Lane 4: E. coli lysate (15 µL/Lane)
Predicted band size: 55 kDa
Observed band size: 65 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1307-1) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"