Lin28B Mouse Monoclonal Antibody [A2-4-5]
Catalog# M1312-1
Lin28B Mouse Monoclonal Antibody [A2-4-5]
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
Lin28B Mouse Monoclonal Antibody [A2-4-5]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human Lin28B aa 73-250 / 250.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 27 kDa
Positive Control
JAR cell lysate, NCCIT, human placenta tissue.
Conjugation
unconjugated
Clone Number
A2-4-5
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100-1:200
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IHC-P
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1:1,000
Target
Function
LIN28 is thought to regulate the self-renewal of stem cells. In nematodes, the LIN28 homolog lin-28 is a heterochronic gene that determines the onset of early larval stages of developmental events in Caenorhabditis elegans, by regulating the self-renewal of nematode stem cells in the skin (called seam cells) and vulva (called VPCs) during development. In mice, LIN28 is highly expressed in mouse embryonic stem cells and during early embryogenesis.It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Lin28B is high expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC).
Background References
1. "TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation." Heo I., Joo C., Kim Y.-K., Ha M., Yoon M.-J., Cho J., Yeom K.-H., Han J., Kim V.N. Cell 138:696-708(2009)
2. "Lin28 promotes transformation and is associated with advanced human malignancies." Viswanathan S.R., Powers J.T., Einhorn W., Hoshida Y., Ng T.L., Toffanin S., O\'Sullivan M., Lu J., Phillips L.A., Lockhart V.L., Shah S.P., Tanwar P.S., Mermel C.H., Beroukhim R., Azam M., Teixeira J., Meyerson M., Hughes T.P. Daley G.Q. Nat. Genet. 41:843-848(2009)
3. "The Lin28 cold-shock domain remodels pre-let-7 microRNA." Mayr F., Schutz A., Doge N., Heinemann U. Nucleic Acids Res. 40:7492-7506(2012)
Sequence Similarity
Belongs to the lin-28 family.
Tissue Specificity
Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver. Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). Tends to be up-regulated in triple-negative (ER-,PR-,HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas.
Subcellular Location
Cytoplasm, nucleus
UNIPROT
Synonyms
CSDD 2 antibody
CSDD2 antibody
FLJ16517 antibody
Lin 28 homolog B (C. elegans) antibody
Lin 28.2 antibody
Lin-28B antibody
LIN28B antibody
LN28B_HUMAN antibody
Protein lin-28 homolog B antibody
Images
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☑ Relative expression (RE)
Western blot analysis of Lin28B on different lysates with Mouse anti-Lin28B antibody (M1312-1) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (negative)
Lane 2: JAR cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1312-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining Lin28B in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. and counterstained with DAPI in order to highlight the nucleus (blue).
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Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-Lin28B antibody (M1312-1) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1312-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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