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ERAS Mouse Monoclonal Antibody [D10-B9]

Usd: 350

Specification

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Catalog# M1505-1

ERAS Mouse Monoclonal Antibody [D10-B9]

Application

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

ERAS Mouse Monoclonal Antibody [D10-B9]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human ERAS aa 1-233 / 233.

Species Reactivity

Human, Mouse

Validated Applications

IF-Cell, IHC-P

Molecular Weight

Predicted band size: 25 kDa

Positive Control

Human colon cancer tissue, human brain tissue, mouse brain tissue, NCCIT.

Conjugation

unconjugated

Clone Number

D10-B9

RRID

AB_3073100

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Cell

  • 1:50

  • IHC-P

  • 1:200-1:1,000

Target

Function

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells (By similarity). Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Background References

1. Takahashi K et al. Role of Eras in promoting tumor-like properties in mouse embryonic stem cells. Nature 423:541-545 (2003).

2. Liu Y et al. Role of the ERas gene in gastric cancer cells. Oncol Rep 30(1):50-6 (2013).

3. Kubota E et al. Role of ES cell-expressed Ras (ERas) in tumorigenicity of gastric cancer. Am J Pathol 177(2):955-63 (2010).

Sequence Similarity

Belongs to the small GTPase superfamily. Ras family.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q7Z444 Human

SwissProt: Q7TN89 Mouse

Synonyms

E ras antibody

E-Ras antibody

ecat5 antibody

Embryonic stem cell expressed Ras antibody

Embryonic stem cell-expressed Ras antibody

Eras antibody

ES cell expressed Ras antibody

GTPase ERas antibody

Ha-Ras2 antibody

HRAS2 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ERAS antibody (<a href="/products/M1505-1" style="font-weight: bold;text-decoration: underline;">M1505-1</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1505-1" style="font-weight: bold;text-decoration: underline;">M1505-1</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ERAS antibody (M1505-1) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ERAS antibody (<a href="/products/M1505-1" style="font-weight: bold;text-decoration: underline;">M1505-1</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1505-1" style="font-weight: bold;text-decoration: underline;">M1505-1</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ERAS antibody (M1505-1) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ERAS antibody (<a href="/products/M1505-1" style="font-weight: bold;text-decoration: underline;">M1505-1</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1505-1" style="font-weight: bold;text-decoration: underline;">M1505-1</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ERAS antibody (M1505-1) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ICC staining ERAS in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ERAS monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc&trade; 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.

    ICC staining ERAS in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ERAS monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"