Lamin B2 Mouse Monoclonal Antibody [J9-C4]
Catalog# M1505-10
Lamin B2 Mouse Monoclonal Antibody [J9-C4]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
Lamin B2 Mouse Monoclonal Antibody [J9-C4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human Lamin B2 aa 1-50 / 620.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 70 kDa
Positive Control
Jurkat cell lysates, human lung cancer tissue.
Conjugation
unconjugated
Clone Number
J9-C4
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IHC-P
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1:500-1:2,000
Target
Function
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Background References
1. Li Y et al. The role of lamin B2 in human diseases. Gene. 2023 Jun
2. Payumo AY et al. Lamin B2, Guardian of Cardiomyocyte Nuclear Division. Dev Cell. 2020 Apr
Sequence Similarity
Belongs to the intermediate filament family.
Post-translational Modification
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Subcellular Location
Nucleus lamina.
UNIPROT
Synonyms
Lamin-B2 antibody
LMN 2 antibody
LMN B2 antibody
LMN2 antibody
LMNB 2 antibody
LMNB2 antibody
LMNB2_HUMAN antibody
MGC2721 antibody
RGD1563803 antibody
Images
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Western blot analysis on Jurkat cell lysates using anti- Lamin B2 mouse mAb.
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-Lamin B2 antibody (M1505-10) at 1/1000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-10) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Lamin B2 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Lamin B2 Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human
Conjugate: unconjugated
