GRP78 / BIP Mouse Monoclonal Antibody [2-7]
Catalog# M1505-13
GRP78 / BIP Mouse Monoclonal Antibody [2-7]
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WB
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IF-Cell
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IHC-P
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IF-Tissue
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FC
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Human
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Mouse
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Rat
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Zebrafish
Predicted species support after-sales service
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unconjugated
Overview
Product Name
GRP78 / BIP Mouse Monoclonal Antibody [2-7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human GRP78 aa 605-654 / 654.
Species Reactivity
Human, Mouse, Rat (Predicted: Zebrafish)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IHC-P, IF-Tissue, FC
Molecular Weight
Predicted band size: 78 kDa
Positive Control
L-929 cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate, mouse liver tissue lysate, rat liver tissue lysate, rat pancreas tissue lysate, Hela, hybrid fish (crucian-carp) heart tissue lysates.
Conjugation
unconjugated
Clone Number
2-7
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IF-Cell
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1:250
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IHC-P
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1:200-1,000
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IF-Tissue
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1:50-1:200
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FC
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1:1,000
Target
Function
Binding immunoglobulin protein (BiP) also known as 78 kDa glucose-regulated protein (GRP-78) or heat shock 70 kDa protein 5 (HSPA5) is a protein that in humans is encoded by the HSPA5 gene. BiP is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. Like many stress and heat shock proteins, BiP/GRP78 has potent immunological activity when released from the internal environment of the cell into the extracelluar space.specifically, it feeds anti-inflammatory and pro-resolutory signals into immune networks, thus helping to resolve inflammation.
Background References
1. Human XTP3-B forms an endoplasmic reticulum quality control scaffold with the HRD1-SEL1L ubiquitin ligase complex and BiP." Hosokawa N., Wada I., Nagasawa K., Moriyama T., Okawa K., Nagata K. J. Biol. Chem. 283:20914-20924(2008)
2. Crystal structures of the ATPase domains of four human Hsp70 isoforms: HSPA1L/Hsp70-hom, HSPA2/Hsp70-2, HSPA6/Hsp70B\', and HSPA5/BiP/GRP78." Wisniewska M., Karlberg T., Lehtio L., Johansson I., Kotenyova T., Moche M., Schuler H. PLoS ONE 5:E8625-E8625(2010)
3. Adenosine-derived inhibitors of 78 kDa glucose regulated protein (Grp78) ATPase: insights into isoform selectivity." Macias A.T., Williamson D.S., Allen N., Borgognoni J., Clay A., Daniels Z., Dokurno P., Drysdale M.J., Francis G.L., Graham C.J., Howes R., Matassova N., Murray J.B., Parsons R., Shaw T., Surgenor A.E., Terry L., Wang Y., Wood M., Massey A.J. J. Med. Chem. 54:4034-4041(2011)
Sequence Similarity
Belongs to the heat shock protein 70 family.
Post-translational Modification
AMPylated by FICD. In unstressed cells, AMPylation at Thr-518 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins (By similarity). In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity (By similarity).
Subcellular Location
Cytoplasm, endoplasmic reticulum lumen
Synonyms
78 kDa glucose regulated protein antibody
78 kDa glucose-regulated protein antibody
AL022860 antibody
AU019543 antibody
BIP antibody
D2Wsu141e antibody
D2Wsu17e antibody
Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
Epididymis secretory sperm binding protein Li 89n antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of GRP78 / BIP on different lysates with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/1,000 dilution.
Lane 1: L-929 cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate
Lane 5: Mouse liver tissue lysate
Lane 6: Rat liver tissue lysate
Lane 7: Rat pancreas tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1505-13) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of L-929 cells labeling GRP78 / BIP with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of RAW264.7 cells labeling GRP78 / BIP with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of PC-12 cells labeling GRP78 / BIP with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-13) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-13) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-13) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-13) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of GRP78 / BIP on hybrid fish (crucian-carp) heart tissue lysate using anti-GRP78 / BIP antibody at 1/500 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Characteristics of endoplasmic reticulum stress changes during the differentiation of adipose-derived stromal cells into neurons
Journal: Cytotechnology
DOI: 10.1007/s10616-025-00891-8
IF: 1.7
Application: IHC,WB
Reactivity: Human
Publish date: 2026 Jan
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Study on liver injury caused by lipid accumulation induced by PM2.5 through IRE1α-XBP1s pathway of endoplasmic reticulum stress in mice
Journal: Journal of Environmental Sciences
DOI: 10.1016/j.jes.2026.02.006
IF: 6.3
Application: WB
Reactivity: Mouse
Publish date: 2026 Feb
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Transketolase regulates endoplasmic reticulum stress independent of enzymatic activity in human retinal Müller cells
Journal: Experimental Eye Research
DOI: 10.1016/j.exer.2025.110732
IF: 2.7
Application: WB,IF-cell
Reactivity: Human
Publish date: 2025 Nov
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Cyanidin-3-O-Glucoside Mitigates Hepatotoxicity Induced by 2-Amino-3-Methylimidazo[4,5-f]Quinoline via Endogenous and Exogenous Apoptotic Signaling Pathways: Evidence From In Vivo and In Silico Studies
Journal: Molecular Nutrition & Food Research
DOI: 10.1002/mnfr.70363
IF: 4.2
Application: WB
Reactivity: Mouse
Publish date: 2025 Dec
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