Hsp27 Mouse Monoclonal Antibody [10-8]
Catalog# M1505-3
Hsp27 Mouse Monoclonal Antibody [10-8]
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Hsp27 Mouse Monoclonal Antibody [10-8]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human HSP27 aa 11-190 / 205.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 23 kDa
Positive Control
HeLa cell lysate, MCF7 cell lysate, PC-12 cell lysate, human breast carcinoma tissue, mouse esophagus tissue, rat esophagus tissue, Hela.
Conjugation
unconjugated
Clone Number
10-8
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:50
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IHC-P
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1:200-1:1,000
Target
Function
Hsp27 is a chaperone of the sHsp(small heat shock protein) group among ubiquitin, α-crystallin,Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and?cell differentiation. They also take part in signal transduction. The main function of Hsp27 is to provide thermotolerance in vivo, cytoprotection, and support of cell survival under stress conditions. More specialized functions of Hsp27 are manifold and complex. In vitro it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding by the Hsp70-complex. Hsp27 is also involved in the apoptotic signalling pathway. Hsp27 interacts with the outer mitochondrial membranes and interferes with the activation of?cytochrome c/Apaf-1/dATP complex and therefore inhibits the activation of procaspase-9.
Background References
1. Heat shock protein 27 is associated with freedom from graft vasculopathy after human cardiac transplantation." De Souza A.I.,Wait R.,Mitchell A.G.,Banner N.R.,Dunn M.J.,Rose M.L. Circ. Res. 97:192-198(2005)
2. PKA-induced F-actin rearrangement requires phosphorylation of Hsp27 by the MAPKAP kinase MK5." Kostenko S.,Johannessen M.,Moens U. Cell. Signal. 21:712-718(2009)
3. The mutational spectrum in a cohort of Charcot-Marie-Tooth disease type 2 among the Han Chinese in Taiwan." Lin K.P., Soong B.W.,Yang C.C.,Huang L.W.,Chang M.H., Lee I.H.,Antonellis A.,Lee Y.C. PLoS ONE 6:E29393-E29393(2011)
Sequence Similarity
Belongs to the small heat shock protein (HSP20) family.
Tissue Specificity
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Post-translational Modification
Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.
Subcellular Location
Cytoplasm, nucleus
Synonyms
Heat shock 27kDa protein antibody
28 kDa heat shock protein antibody
CMT2F antibody
DKFZp586P1322 antibody
epididymis secretory protein Li 102 antibody
Estrogen regulated 24 kDa protein antibody
Estrogen-regulated 24 kDa protein antibody
Heat shock 25kDa protein 1 antibody
Heat shock 27 kDa protein antibody
Heat shock 27kD protein 1 antibody
ExpandImages
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Western blot analysis of Hsp27 on different lysates with Mouse anti-Hsp27 antibody (M1505-3) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: PC-12 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1505-3) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Hsp27 antibody (M1505-3) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-3) at 1/1.000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue with Mouse anti-Hsp27 antibody (M1505-3) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Mouse anti-Hsp27 antibody (M1505-3) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of Hela cells labeling Hsp27 with Mouse anti-Hsp27 antibody (M1505-3) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Hsp27 antibody (M1505-3) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Molecular mechanisms by which Ac-SDKP regulates HSP27 via the p38MAPK/mTOR autophagy pathway to ameliorate pulmonary fibrosis
Journal: CELLULAR SIGNALLING
DOI: 10.1016/j.cellsig.2025.112151
IF: 3.7
Application: IF-tissue
Reactivity: Rat
Publish date: 2025 Sept
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